RAB14
RAB14 (Ras-related protein Rab-14, also known as FBP or RAB-14) is a small GTPase of the Rab family that regulates intracellular membrane trafficking. It cycles between GDP-bound and GTP-bound states and helps move cargo between the Golgi complex and endosomes. Browse Assay Genie's current range of RAB14 research reagents below, or read on for the biology behind the target.
Rab proteins are key regulators of intracellular membrane trafficking, acting from the formation of transport vesicles through to their fusion with target membranes. RAB14 behaves as a molecular switch. In its inactive GDP-bound form it is not engaged, while in its active GTP-bound form it recruits a set of downstream effectors to membranes. These effectors are directly responsible for vesicle formation, movement, tethering and fusion.
RAB14 is involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. It regulates Golgi-to-endosome transport of FGFR-containing vesicles, a process important for developing the basement membrane and the epiblast and primitive endoderm lineages in early postimplantation development. It may act by modulating the association of the kinesin KIF16B with cargo on endosomes. Together with its guanine nucleotide exchange factor DENND6A, RAB14 regulates the endocytic transport of ADAM10, N-cadherin (CDH2) shedding and cell-cell adhesion. Through interaction with RUFY1 and RAB4B it mediates endosomal tethering and fusion, and its interaction with RAB11FIP1 may contribute to neurite formation.
The RAB14 protein belongs to the small GTPase superfamily and the Rab family, and carries GTPase activity (EC 3.6.5.2). It is localised to the recycling endosome, early endosome membrane, Golgi apparatus and trans-Golgi network membranes, and phagosomes. The RAB14 gene is listed by HGNC within the RAB, member RAS oncogene GTPases group. Mouse and rat both have a Rab14 ortholog, which supports comparative work across model systems.
RAB14 regulates membrane trafficking between the Golgi complex and endosomes, and mediates endosomal tethering and fusion with RUFY1 and RAB4B.
RAB14 controls Golgi-to-endosome transport of FGFR-containing vesicles, important for basement membrane and epiblast and primitive endoderm lineages.
With DENND6A, RAB14 regulates endocytic transport of ADAM10, N-cadherin shedding and cell-cell adhesion.
Interaction of RAB14 with RAB11FIP1 may function in the process of neurite formation.
RAB14 is a small GTPase that regulates membrane trafficking between the Golgi complex and endosomes. In its GTP-bound state it recruits effectors that drive vesicle formation, movement, tethering and fusion. It also regulates FGFR-vesicle transport in early development and ADAM10 endocytic transport.
The official HGNC name is RAB14, member RAS oncogene family, and UniProt lists the protein as Ras-related protein Rab-14. Aliases include FBP and RAB-14. The gene belongs to the RAB, member RAS oncogene GTPases group.
Yes. Mouse and rat each have a Rab14 ortholog, and RAB14 belongs to the Rab family of the small GTPase superfamily. Rab GTPases act as GDP/GTP-regulated switches in membrane trafficking. Check the datasheet of any product for its validated reactivity.
Other Rab family GTPases and trafficking regulators involved in endosomal and Golgi transport.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P61106; product specifications from Assay Genie datasheets.