MAT1A
MAT1A (methionine adenosyltransferase 1A) is an enzyme that catalyses the formation of S-adenosylmethionine from methionine and ATP. It is also known as S-adenosylmethionine synthase isoform type-1, MAT, SAMS, MATA1, SAMS1 and MAT-I/III. Browse Assay Genie's current range of MAT1A research reagents below, or read on for the biology behind the target.
MAT1A catalyses a two-step reaction in which the adenosyl moiety of ATP is transferred to methionine. The first step forms S-adenosylmethionine (AdoMet) and triphosphate; the triphosphate is then hydrolysed, and the same enzyme catalyses both steps. The triphosphate is cleaved to pyrophosphate and inorganic phosphate. S-adenosylmethionine is the source of methyl groups for most biological methylations, which places the MAT1A protein at a central point in cellular methylation metabolism.
The protein belongs to the AdoMet synthase family, and HGNC places the MAT1A gene in the methionine adenosyltransferase family. The encoded protein is found as a homotetramer, known as MAT I, or as a homodimer, known as MAT III. A third form, MAT II (gamma), is encoded by a different gene, MAT2A. The two assemblies of MAT1A explain the combined alias MAT-I/III. The UniProt entry for the human protein is Q00266, and mouse and rat each have a Mat1a ortholog.
The gene was previously named methionine adenosyltransferase I, alpha, and is also listed under the aliases MAT, SAMS, MATA1 and SAMS1. Mutations in the MAT1A gene are associated with methionine adenosyltransferase deficiency, which makes it a reference point for work on inherited disorders of methionine metabolism. Researchers studying methionine metabolism often examine MAT1A alongside MAT2A and MAT2B.
MAT1A catalyses the conversion of methionine and ATP to S-adenosylmethionine, making it a core enzyme for studying methionine utilisation.
S-adenosylmethionine is the source of methyl groups for most biological methylations, so MAT1A is relevant to studies of methyl donor supply.
Mutations in the MAT1A gene are associated with methionine adenosyltransferase deficiency, supporting research into this inherited disorder.
MAT1A forms MAT I and MAT III assemblies, while MAT II is encoded by MAT2A, allowing comparison of methionine adenosyltransferase forms.
MAT1A catalyses the formation of S-adenosylmethionine from methionine and ATP in two steps: AdoMet and triphosphate are formed, then the triphosphate is hydrolysed. S-adenosylmethionine is the source of methyl groups for most biological methylations.
MAT1A is also called S-adenosylmethionine synthase isoform type-1, MAT, SAMS, MATA1, SAMS1 and MAT-I/III, and was previously named methionine adenosyltransferase I, alpha. The protein occurs as a homotetramer (MAT I) or a homodimer (MAT III).
MAT2A encodes a third methionine adenosyltransferase form, MAT II (gamma), distinct from the MAT I and MAT III forms of MAT1A. Mutations in MAT1A are associated with methionine adenosyltransferase deficiency. Mouse and rat both have a Mat1a ortholog; check the datasheet of any product for its validated reactivity.
Other methionine adenosyltransferase family members and similarly named targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q00266; product specifications from Assay Genie datasheets.