DAPK1
DAPK1 (death associated protein kinase 1, also known as DAPK) is a calmodulin-dependent serine/threonine kinase and a positive mediator of gamma-interferon induced programmed cell death. It is a tumour suppressor candidate and the founding member of the DAP kinase subfamily. Browse Assay Genie's current range of DAPK1 research reagents below, or read on for the biology behind the target.
DAPK1 is a positive mediator of gamma-interferon induced programmed cell death. As a serine/threonine kinase (EC 2.7.11.1) it depends on calmodulin for activity. It localises to the cytoplasm and the cytoskeleton. Studies of the isoforms show that they do not behave identically: isoform 2 cannot induce apoptosis but can induce membrane blebbing, so the isoforms are worth distinguishing in experimental design.
The DAPK1 gene encodes a structurally unusual protein of about 160 kDa. It carries 8 ankyrin repeats and 2 putative P-loop consensus sites, which is why HGNC places it in the ankyrin repeat domain containing group as well as the death associated protein kinases and ROCO families. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the DAP kinase subfamily. Alternative splicing produces multiple transcript variants. The mouse ortholog is Dapk1.
DAPK1 is regarded as a tumour suppressor candidate, reflecting its role in promoting cell death. The symbol DAPK is a previous symbol for the gene, and the protein is also named death-associated protein kinase 1 in UniProt. Researchers studying apoptosis, cytoskeletal changes such as membrane blebbing, and cancer biology use DAPK1 as a defined target. Related DAP kinase family members, such as DAPK3, are often considered alongside it when comparing kinase functions.
DAPK1 positively mediates gamma-interferon induced programmed cell death, making it a central kinase for studying cell death signalling.
DAPK1 is a tumour suppressor candidate, so its activity and expression are of interest in cancer biology.
DAPK1 is a calmodulin-dependent serine/threonine kinase of the CAMK family and the DAP kinase subfamily.
Alternative splicing yields multiple variants, and isoform 2 induces membrane blebbing without inducing apoptosis.
DAPK1 is a calmodulin-dependent serine/threonine kinase that acts as a positive mediator of gamma-interferon induced programmed cell death. It is found in the cytoplasm and cytoskeleton and is considered a tumour suppressor candidate. Check the datasheet of any product for its validated reactivity.
DAPK is a previous symbol, and the protein is named death-associated protein kinase 1. Alternative splicing gives multiple transcript variants. Isoform 2 cannot induce apoptosis but can induce membrane blebbing, which shows that isoforms can differ functionally.
DAPK1 belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the DAP kinase subfamily. HGNC also groups it with ankyrin repeat domain containing proteins and the ROCO family. The mouse ortholog is Dapk1.
Other DAP kinase family members and related cell death regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P53355; product specifications from Assay Genie datasheets.