PLIN3
PLIN3 (perilipin 3) is a member of the perilipin family and a structural component of lipid droplets. Also known as TIP47, PP17 and M6PRBP1, it also supports the return of mannose 6-phosphate receptors from endosomes to the trans-Golgi network. Browse Assay Genie's current range of PLIN3 research reagents below, or read on for the biology behind the target.
PLIN3 is a structural component of lipid droplets and is required for the formation and maintenance of lipid storage droplets. It has a second, separate role in membrane trafficking. It is required for transport of mannose 6-phosphate receptors (MPRs) from endosomes to the trans-Golgi network. This lets the receptors return to the Golgi after they deliver lysosomal hydrolases to endosomes.
The PLIN3 protein interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and it is required for endosome-to-Golgi transport. It also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. This interaction has been shown to increase the affinity of PLIN3 for its cargo. UniProt lists its subcellular locations as lipid droplet, endosome membrane and cytoplasm, which matches its dual role in lipid storage and receptor recycling. Researchers studying the PLIN3 gene should note that multiple transcript variants encoding different isoforms have been found.
PLIN3 belongs to the perilipin family, which HGNC classifies as the Perilipins gene group. The mouse ortholog is Plin3. The gene was previously named mannose-6-phosphate receptor binding protein 1, and the aliases TIP47, PP17 and M6PRBP1 still appear in the literature. Take care to match symbols and names when comparing older papers with current nomenclature.
PLIN3 is a structural component of lipid droplets and is required for the formation and maintenance of lipid storage droplets.
PLIN3 is required for transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.
MPRs deliver lysosomal hydrolases from the Golgi to endosomes, and PLIN3 supports their return to the Golgi for further rounds of sorting.
PLIN3 binds RAB9 directly, and this interaction increases the affinity of PLIN3 for its cargo.
PLIN3 is a structural component of lipid droplets, needed for forming and maintaining lipid storage droplets. It is also required for moving mannose 6-phosphate receptors from endosomes back to the trans-Golgi network, where it interacts with the cytoplasmic domains of the receptors and binds RAB9.
PLIN3 is also called TIP47, PP17 and M6PRBP1. Its previous name was mannose-6-phosphate receptor binding protein 1, and its current HGNC name is perilipin 3. NCBI Gene notes that multiple transcript variants encoding different isoforms have been found.
PLIN3 has a mouse ortholog, Plin3, and belongs to the perilipin family. Check the datasheet of any product for its validated reactivity before using it with a particular species.
Other perilipin family members involved in lipid droplet biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O60664; product specifications from Assay Genie datasheets.