RBBP4
RBBP4 (RB binding protein 4, chromatin remodeling factor), also known as RbAp48, NURF55 and lin-53, is a nuclear WD-repeat protein that binds histones. It serves as a core subunit of several complexes that assemble, remodel and deacetylate chromatin. Browse Assay Genie's current range of RBBP4 research reagents below, or read on for the biology behind the target.
RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates, in a manner regulated by nucleosomal DNA. It is a component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly after DNA replication and DNA repair. It also belongs to the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression.
The RBBP4 protein is also a component of the nucleosome remodeling and histone deacetylase (NuRD) complex, which represses transcription through histone deacetylation and nucleosome remodeling. It is part of the PRC2 complex, which promotes repression of homeotic genes during development, and of the NURF (nucleosome remodeling factor) complex. HGNC groups it with the EMSY, SIN3 histone deacetylase and MuvB complex subunits as well as those of NuRD, PRC2 and NURF. NCBI Gene describes it as part of the Mi-2 complex and of co-repressor complexes that are integral to transcriptional silencing.
The RBBP4 gene encodes a ubiquitously expressed nuclear protein of the highly conserved WD repeat RBAP46/RBAP48/MSI1 family. UniProt also places it at telomeres. It is among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation, and it appears to be involved in repression of E2F-responsive genes, which explains the earlier name retinoblastoma-binding protein 4. Three transcript variants encoding different isoforms have been reported.
RBBP4 is a shared subunit of NuRD, NURF and PRC2, making it useful for studying how remodeling complexes engage histone substrates.
As part of HDAC and co-repressor complexes, RBBP4 contributes to histone deacetylation and transcriptional silencing.
RBBP4 is a component of CAF-1, which is required for chromatin assembly following DNA replication and DNA repair.
RBBP4 binds retinoblastoma protein and appears to contribute to repression of E2F-responsive genes that regulate cell proliferation.
RBBP4 is a core histone-binding subunit of several chromatin complexes. It may target chromatin assembly factors, remodeling factors and histone deacetylases to histones. It is part of CAF-1, HDAC, NuRD, PRC2 and NURF complexes, linking chromatin assembly, remodeling and transcriptional repression.
RBBP4 is also called RbAp48, NURF55 and lin-53. Earlier names were retinoblastoma-binding protein 4 and retinoblastoma binding protein 4. UniProt lists the protein as histone-binding protein RBBP4. Three transcript variants encoding different isoforms have been described for the gene.
NCBI Gene describes RBBP4 as belonging to a highly conserved subfamily of WD-repeat proteins, and the lin-53 alias reflects this. The mouse ortholog is Rbbp4. Check the datasheet of any product for its validated reactivity.
Other chromatin-remodelling, histone deacetylase and Polycomb complex components, and retinoblastoma pathway targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q09028; product specifications from Assay Genie datasheets.