CYP2B6
CYP2B6 (cytochrome P450 family 2 subfamily B member 6, also known as CPB6, CYPIIB6 and CYP2B) is an endoplasmic reticulum monooxygenase of the cytochrome P450 superfamily. It takes part in drug metabolism and is induced by phenobarbital. Browse Assay Genie's current range of CYP2B6 research reagents below, or read on for the biology behind the target.
CYP2B6 belongs to the cytochrome P450 superfamily. Cytochrome P450 proteins are monooxygenases that catalyse many reactions involved in drug metabolism and in the synthesis of cholesterol, steroids and other lipids. CYP2B6 is known to metabolise some xenobiotics, including the anti-cancer drugs cyclophosphamide and ifosphamide. In UniProt, the annotated function describes the allele 2B6*9 variant, which has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
The protein localises to the endoplasmic reticulum, and UniProt records it at the endoplasmic reticulum and microsome membranes. Its expression is induced by phenobarbital, which is reflected in the earlier HGNC names for the gene, such as cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6. The gene is classed in the HGNC group cytochrome P450 family 2 and carries the EC designation 1.14.13.-. Researchers studying the CYP2B6 gene or the CYP2B6 protein often work on xenobiotic metabolism and on variation between alleles.
The CYP2B6 gene lies in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. Both the gene and its pseudogene, CYP2B7, sit in the middle of a CYP2A pseudogene. Transcript variants have been described, but it has not been resolved whether they are produced by CYP2B6 or by CYP2B7. This should be borne in mind when designing transcript-level experiments.
CYP2B6 is a monooxygenase involved in drug metabolism, and it metabolises some xenobiotics, making it a relevant enzyme for studying how compounds are processed.
The enzyme is known to metabolise the anti-cancer drugs cyclophosphamide and ifosphamide, which links it to studies of chemotherapeutic agent metabolism.
CYP2B6 expression is induced by phenobarbital, so it is used to study inducible regulation of cytochrome P450 genes.
Allele 2B6*9 shows low affinity for anandamide and produces only 11,12 EpETrE-EAs, an example of how variants alter substrate handling.
CYP2B6 is a cytochrome P450 monooxygenase located at the endoplasmic reticulum. Members of this superfamily catalyse reactions in drug metabolism and in the synthesis of cholesterol, steroids and other lipids. CYP2B6 metabolises some xenobiotics, including cyclophosphamide and ifosphamide.
CYP2B6 is also known as CPB6, CYPIIB6 and CYP2B. Older HGNC names refer to subfamily IIB, phenobarbital-inducible, polypeptide 6. Transcript variants have been described, but it is unresolved whether they arise from CYP2B6 or from the closely related pseudogene CYP2B7.
CYP2B6 lies in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. It is placed in the HGNC cytochrome P450 family 2 group, alongside genes such as CYP2A6 and CYP2D6. Check the datasheet of any product for its validated reactivity.
Other cytochrome P450 family 2 members and drug-metabolising enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P20813; product specifications from Assay Genie datasheets.