BRD8
BRD8 (bromodomain-containing protein 8, also known as SMAP and p120) is a nuclear bromodomain protein thought to act as a coactivator of hormone-activated nuclear receptors. It is a subunit of the Tip60/NuA4 histone acetyltransferase complex and of a SWR1-like histone exchange complex. Browse Assay Genie's current range of BRD8 research reagents below, or read on for the biology behind the target.
BRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors. According to UniProt, isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. NCBI Gene adds that the protein interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements.
At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex, which activates select genes principally by acetylating nucleosomal histones H4 and H2A. This modification may alter nucleosome-DNA interactions and promote binding of the modified histones by other proteins that positively regulate transcription. The complex may be required for transcriptional programmes linked to oncogene and proto-oncogene driven growth induction, tumour suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also act directly in DNA repair when recruited to sites of DNA damage. BRD8 is also a component of a SWR1-like complex that specifically removes histone H2A.Z/H2AZ1 from the nucleosome.
The BRD8 gene encodes a protein containing a bromodomain, and HGNC places it in the bromodomain containing group and among the Tip60/Nua4 histone acetyltransferase complex subunits. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified, which is why isoform-specific roles are described in the literature. The protein is also known as SMAP and p120, and the mouse ortholog is Brd8.
BRD8 is thought to coactivate hormone-dependent transcription, including thyroid hormone receptor signalling through thyroid response elements.
As a NuA4 HAT complex subunit, BRD8 contributes to acetylation of nucleosomal histones H4 and H2A during transcriptional activation.
The NuA4 complex may be required for DNA repair programmes and may act directly when recruited to sites of DNA damage.
BRD8 is a component of a SWR1-like complex that mediates removal of histone H2A.Z/H2AZ1 from the nucleosome.
BRD8 may act as a coactivator for hormone-activated nuclear receptors, including thyroid hormone receptor. It is also a subunit of the NuA4 histone acetyltransferase complex, which acetylates histones H4 and H2A, and of a SWR1-like complex that removes histone H2A.Z from nucleosomes.
BRD8 is the HGNC symbol for bromodomain containing 8, and the protein is also called SMAP and p120. Alternatively spliced transcript variants encode distinct isoforms; UniProt describes isoform 2 as stimulating nuclear receptor activity, and isoforms 1 and 2 as NuA4 components.
A mouse ortholog, Brd8, is recorded. BRD8 belongs to the HGNC bromodomain containing group and the Tip60/Nua4 histone acetyltransferase complex subunits group. Check the datasheet of any product for its validated reactivity.
Other bromodomain-containing proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H0E9; product specifications from Assay Genie datasheets.