CDK11B
CDK11B (cyclin dependent kinase 11B) is a serine/threonine protein kinase of the CDC2/CDKX subfamily that contributes to cell cycle control. It is also known as CDK11-p110, CDK11-p58, CDK11-p46 and CDC2L1, and was formerly named cell division cycle 2-like 1 (PITSLRE proteins). Browse Assay Genie's current range of CDK11B research reagents below, or read on for the biology behind the target.
CDK11B is a member of the serine/threonine protein kinase family, and members of this family are known to be essential for eukaryotic cell cycle control. The UniProt annotation describes an isoform that is specifically expressed during the G2-M phases of the cell cycle. This isoform phosphorylates Ser-2 of the C-terminal domain (CTD) of POLR2A, the large subunit of RNA polymerase II. It also promotes centromeric transcription, which maintains centromeric cohesion during mitosis. Consistent with these roles, the protein is found in the nucleus, on chromosomes and at the centromere, as well as in the cytoplasm.
The CDK11B protein belongs to the protein kinase superfamily, in the CMGC Ser/Thr protein kinase family and the CDC2/CDKX subfamily. HGNC places it in the cyclin dependent kinases gene group. The CDK11B gene can produce several protein forms, and the alias names CDK11-p110, CDK11-p58 and CDK11-p46 reflect this. NCBI Gene notes that alternative splicing results in multiple transcript variants. The kinase can also be cleaved by caspases and may play a role in apoptosis.
Because of a segmental duplication, the CDK11B gene shares very high sequence identity with a neighbouring gene, CDK11A. These two genes are frequently deleted or altered in neuroblastoma. This high similarity is worth keeping in mind when designing experiments, since reagents may not readily distinguish the two products. Orthologues of CDK11B are named Cdk11b in both mouse and rat.
CDK11B is an isoform expressed during G2-M, making it relevant to studies of mitotic progression and cell cycle control.
The kinase phosphorylates Ser-2 of the POLR2A CTD, linking it to RNA polymerase II regulation.
CDK11B promotes centromeric transcription to maintain centromeric cohesion during mitosis, a focus for chromosome segregation studies.
The kinase can be cleaved by caspases, and its gene is frequently deleted or altered in neuroblastoma.
CDK11B is a serine/threonine protein kinase involved in cell cycle control. An isoform expressed during G2-M phosphorylates Ser-2 of the RNA polymerase II CTD on POLR2A and promotes centromeric transcription, which maintains centromeric cohesion during mitosis.
The gene was formerly called cell division cycle 2-like 1 (CDC2L1), and the PITSLRE proteins are named after it. CDK11-p110, CDK11-p58 and CDK11-p46 are protein forms, and alternative splicing yields multiple transcript variants.
A segmental duplication gives CDK11B very high sequence identity with a neighbouring gene, CDK11A. Both genes are frequently deleted or altered in neuroblastoma. The mouse and rat orthologues are Cdk11b; check the datasheet of any product for its validated reactivity.
Other cyclin dependent kinases, the closely related CDK11A and cell cycle inhibitors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P21127; product specifications from Assay Genie datasheets.