PREP
PREP (prolyl endopeptidase) is a cytosolic peptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within short peptides. It belongs to the peptidase S9A family and is classified as EC 3.4.21.26. Browse Assay Genie's current range of PREP research reagents below, or read on for the biology behind the target.
PREP encodes prolyl endopeptidase, an enzyme that cleaves peptide bonds on the C-terminal side of prolyl residues. Its substrates are peptides rather than large folded proteins: the enzyme acts within peptides up to approximately 30 amino acids long. This size restriction defines its role as a processor of short peptides, and it distinguishes the enzyme from general proteases that degrade full-length proteins.
The PREP protein is cytosolic, and UniProt records its subcellular location as the cytoplasm. It belongs to the peptidase S9A family and carries the EC number 3.4.21.26. The human gene is orthologous to Prep in both mouse and rat, which allows the enzyme to be studied across common rodent model systems.
According to the RefSeq summary, prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. For this reason, the PREP gene and its protein product are of interest to researchers studying peptide processing and turnover. The HGNC approved name is prolyl endopeptidase, and no alternative aliases or previous symbols are listed for the gene.
PREP has been reported to participate in the maturation and degradation of peptide hormones, making it relevant to studies of peptide processing.
Prolyl endopeptidases are reported to act on neuropeptides, so PREP is studied in work on how short signalling peptides are turned over.
PREP cleaves after prolyl residues in peptides up to about 30 amino acids, providing a defined model for prolyl-directed proteolysis.
As a member of the peptidase S9A family, PREP is used to examine the activity and biology of this group of serine peptidases.
PREP is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides up to approximately 30 amino acids long. It has been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.
The HGNC approved name is prolyl endopeptidase, and UniProt uses the same protein name. No aliases or previous symbols are listed. The enzyme is classified as EC 3.4.21.26 and belongs to the peptidase S9A family.
Yes. Mouse and rat each have an ortholog named Prep, so the enzyme can be studied in rodent models. Check the datasheet of any product for its validated reactivity.
Other peptidases, peptide hormone and neuropeptide targets that may be of interest alongside PREP.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P48147; product specifications from Assay Genie datasheets.