REC8
REC8 (meiotic recombination protein REC8 homolog, also known as Rec8p, kleisin-alpha or REC8L1) is a kleisin-family component of the meiotic cohesin complex. It is required during meiosis for the separation of sister chromatids and homologous chromosomes. Browse Assay Genie's current range of REC8 research reagents below, or read on for the biology behind the target.
REC8 is required during meiosis for the separation of sister chromatids and of homologous chromosomes. Its function is controlled by proteolytic cleavage. During anaphase I, separin cleaves REC8 on chromosome arms, which allows homologous chromosomes to separate in meiosis I. During anaphase II, cleavage of REC8 at the centromeres allows sister chromatids to separate in meiosis II.
The REC8 gene encodes a member of the kleisin family of SMC (structural maintenance of chromosomes) protein partners, and the protein belongs to the rad21 family. HGNC places it in the cohesin complex and kleisin gene groups. The protein is found in the nucleus and on chromosomes, including the centromere. During meiosis it localises to the axial elements of chromosomes in both oocytes and spermatocytes. Multiple alternatively spliced transcript variants have been described for the REC8 gene, and they encode the same protein.
In the mouse, where the ortholog is also named Rec8, the homologous protein is a key component of the meiotic cohesion complex. That complex regulates sister chromatid cohesion and recombination between homologous chromosomes. The gene was previously named REC8-like 1 (yeast) and REC8 homolog (yeast), after the yeast meiotic recombination protein from which it takes its name.
REC8 is required for separation of sister chromatids and homologous chromosomes, making it a central protein for studying how meiotic divisions proceed.
As a kleisin partner of SMC proteins in the meiotic cohesion complex, REC8 is used to study how cohesion between sister chromatids is established and released.
The meiotic cohesion complex containing REC8 regulates recombination between homologous chromosomes, so the protein is relevant to studies of crossover and pairing.
REC8 localises to chromosome axial elements in oocytes and spermatocytes, which makes it a useful protein for investigating gametogenesis.
REC8 is a meiosis-specific cohesin kleisin required for separation of sister chromatids and homologous chromosomes. Separin cleaves it on chromosome arms in anaphase I and at centromeres in anaphase II, which allows the two successive meiotic divisions to proceed.
REC8 is also called Rec8p, kleisin-alpha and REC8L1. Earlier names include REC8-like 1 (yeast) and REC8 homolog (yeast). UniProt lists it as meiotic recombination protein REC8 homolog. Multiple splice variants of the gene encode the same protein.
Yes. The mouse and rat both have a Rec8 ortholog, and the name derives from the yeast meiotic recombination protein. In the mouse, the homologous protein is a key component of the meiotic cohesion complex. Check the datasheet of any product for its validated reactivity.
Other proteins with products at Assay Genie that are linked by name or by cohesin and chromosome biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O95072; product specifications from Assay Genie datasheets.