NSF
NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase) is a cytoplasmic AAA ATPase required for vesicle-mediated transport. Also known as SKD2 and SEC18, it catalyses the fusion of transport vesicles within the Golgi cisternae. Browse Assay Genie's current range of NSF research reagents below, or read on for the biology behind the target.
NSF is required for vesicle-mediated transport. It catalyses the fusion of transport vesicles within the Golgi cisternae and is also required for transport from the endoplasmic reticulum to the Golgi stack. UniProt annotation describes it as a fusion protein needed for delivery of cargo proteins to all compartments of the Golgi stack, independent of vesicle origin. As an ATPase (EC 3.6.4.6), it couples nucleotide hydrolysis to its role in membrane trafficking.
The human NSF gene encodes the protein vesicle-fusing ATPase, which belongs to the AAA ATPase family and sits within the HGNC AAA ATPases gene group. Orthologues are annotated in mouse and rat as Nsf. The protein is found in the cytoplasm; NCBI Gene reports it in the cytosol and at the plasma membrane. It is annotated with PDZ domain binding and ionotropic glutamate receptor binding activity.
NSF also influences receptor trafficking. Its interaction with the AMPA receptor subunit GRIA2 affects GRIA2 membrane cycling, and NCBI Gene lists involvement in intracellular protein transport, positive regulation of receptor recycling and positive regulation of protein catabolic process. The name derives from its sensitivity to N-ethylmaleimide, and SEC18 and SKD2 are used as alternative names. Disease associations recorded for the NSF gene include cocaine dependence and developmental and epileptic encephalopathy 96.
NSF catalyses vesicle fusion in the Golgi and is needed for endoplasmic reticulum to Golgi transport, making it a core component of secretory pathway studies.
Interaction with GRIA2 influences AMPA receptor membrane cycling, and NSF is annotated with positive regulation of receptor recycling.
NSF is implicated in developmental and epileptic encephalopathy 96, linking vesicle fusion machinery to epilepsy-related disorders.
NSF is implicated in cocaine dependence, and its glutamate receptor binding activity is relevant to synaptic mechanisms of drug response.
NSF is a vesicle-fusing ATPase required for vesicle-mediated transport. It catalyses the fusion of transport vesicles within the Golgi cisternae and is needed for transport from the endoplasmic reticulum to the Golgi stack, delivering cargo proteins to all Golgi compartments.
NSF stands for N-ethylmaleimide sensitive factor, vesicle fusing ATPase. Aliases include SKD2 and SEC18, and earlier names were N-ethylmaleimide-sensitive factor. UniProt lists the protein as vesicle-fusing ATPase, with the EC number 3.6.4.6.
Mouse and rat both carry an orthologous Nsf gene, so rodent models are commonly used. In humans, NSF is implicated in cocaine dependence and developmental and epileptic encephalopathy 96. Check the datasheet of any product for its validated reactivity.
Pathway partners and trafficking-related targets connected to NSF, including AMPA receptor interactors, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P46459; product specifications from Assay Genie datasheets.