MCM5
MCM5 (minichromosome maintenance complex component 5, also known as CDC46) is a subunit of the MCM2-7 complex, the replicative helicase that is essential for DNA replication in eukaryotic cells. It is a nuclear, chromatin-associated member of the MCM family. Browse Assay Genie's current range of MCM5 research reagents below, or read on for the biology behind the target.
MCM5 acts as a component of the MCM2-7 complex, the replicative helicase required for 'once per cell cycle' initiation and elongation of DNA replication in eukaryotic cells. It is a core component of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and around which the replisome is built.
The active ATPase sites in the MCM2-7 ring are formed at the interfaces between two neighbouring subunits. A conserved arginine finger motif is supplied in trans, relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase sites are likely to contribute differentially to the helicase activity of the complex, so each subunit, including MCM5, occupies a defined position in the ring.
The MCM5 gene encodes a protein that belongs to the MCM family of chromatin-binding proteins and can interact with at least two other family members. HGNC places it in the MCM family and the minichromosome maintenance 2-7 complex group. The protein is structurally very similar to the CDC46 protein of S. cerevisiae, which is involved in the initiation of DNA replication, and this is the origin of the alias CDC46. According to the NCBI Gene summary, MCM5 is upregulated in the transition from G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. A mouse ortholog, Mcm5, is recognised.
MCM5 is part of the MCM2-7 helicase needed for once-per-cell-cycle replication initiation and elongation, making it a useful subunit for studying replication licensing.
As a core component of the CDC45-MCM-GINS helicase, MCM5 is relevant to work on how template DNA is unwound and the replisome assembled.
MCM5 is upregulated in the transition from G0 to G1/S and may participate in cell cycle regulation, supporting studies of cell cycle entry.
Arginine finger and Walker A interactions between neighbouring subunits form the ATPase sites of the MCM ring, a focus for mechanistic studies of helicase activity.
MCM5 is a subunit of the MCM2-7 complex, the replicative helicase essential for initiating and elongating DNA replication once per cell cycle. It is a core component of the CDC45-MCM-GINS helicase, which unwinds template DNA and around which the replisome is built.
MCM5 stands for minichromosome maintenance complex component 5, and its UniProt name is DNA replication licensing factor MCM5. The alias CDC46 reflects its similarity to the S. cerevisiae CDC46 protein. Earlier HGNC names referred to minichromosome maintenance deficient 5 (cell division cycle 46).
Yes. MCM5 is structurally very similar to yeast CDC46, which is involved in replication initiation, and a mouse ortholog, Mcm5, exists. It belongs to the MCM family of chromatin-binding proteins. Check the datasheet of any product for its validated reactivity.
Other MCM2-7 helicase subunits and MCM3-associated protein, covering the replication licensing machinery.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P33992; product specifications from Assay Genie datasheets.