EHD1
EHD1 (EH domain containing 1, also known as H-PAST or PAST1) is an ATP- and membrane-binding protein that controls membrane reorganisation and tubulation on ATP hydrolysis. It acts in endocytic membrane fusion and in the trafficking of recycling endosomes. Browse Assay Genie's current range of EHD1 research reagents below, or read on for the biology behind the target.
EHD1 is an ATP- and membrane-binding protein that controls membrane reorganisation and tubulation upon ATP hydrolysis. In vitro it causes vesiculation of endocytic membranes. In cells it acts in early endocytic membrane fusion and in membrane trafficking of recycling endosomes. It is recruited to endosomal membranes upon nerve growth factor stimulation and indirectly regulates neurite outgrowth, and it also plays a role in myoblast fusion.
In neurons, EHD1 is involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons, implicating a function in neuronal APP processing. EHD1 also contributes to cilium biogenesis. It plays a role in forming the ciliary vesicle, an early step in which distal appendage vesicles fuse; this is proposed to depend on EHD1 recruiting the SNARE complex component SNAP29. EHD1 is needed to recruit the transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and IFT20, before Rab8-dependent ciliary membrane extension. It is also required for the loss of CCP110 from the mother centriole, which is essential for basal body maturation.
The EHD1 gene belongs to a highly conserved family encoding EPS15 homology (EH) domain-containing proteins. The EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor, and is an important motif in protein-protein interactions and intracellular sorting. EHD1 is thought to play a role in the endocytosis of IGF1 receptors. The protein belongs to the EHD subfamily of the dynamin/Fzo/YdjA family within the dynamin-like GTPase superfamily, and HGNC places it in the EF-hand domain containing group. Alternatively spliced transcript variants have been described. Localisation is reported at recycling and early endosome membranes, the cell membrane and the ciliary membrane.
EHD1 acts in early endocytic membrane fusion and recycling endosome trafficking, making it a reference protein for studying membrane transport.
EHD1 supports ciliary vesicle formation, recruitment of transition zone proteins and basal body maturation during ciliogenesis.
EHD1 is involved in BACE1 transport and sorting, linking it to neuronal APP processing and to neurite outgrowth after NGF stimulation.
EHD1 plays a role in myoblast fusion, offering a route into membrane remodelling during muscle cell development.
EHD1 binds ATP and membranes and drives membrane tubulation and reorganisation upon ATP hydrolysis. It acts in early endocytic membrane fusion and recycling endosome trafficking, and it contributes to ciliary vesicle formation, myoblast fusion and neuronal BACE1 transport. Check the datasheet of any product for its validated reactivity.
EHD1 is the HGNC symbol for EH domain containing 1, and the protein is also called EH domain-containing protein 1. Aliases and previous symbols include H-PAST, HPAST1, PAST1, FLJ42622 and FLJ44618. Alternatively spliced transcript variants of the gene have been reported.
The EH domain gene family is highly conserved. EHD1 has orthologues in mouse and rat, both named Ehd1. EHD1 belongs to the EHD subfamily of the dynamin-like GTPase superfamily, and a related family member with its own page is EHD3.
Other EH domain-containing proteins and endocytic trafficking targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H4M9; product specifications from Assay Genie datasheets.