OPLAH
OPLAH (5-oxoprolinase, ATP-hydrolysing, also known as OPLA or 5-Opase) is a cytosolic enzyme of the oxoprolinase family. It cleaves 5-oxo-L-proline to L-glutamate, coupling the reaction to ATP hydrolysis. Browse Assay Genie's current range of OPLAH research reagents below, or read on for the biology behind the target.
OPLAH catalyses the cleavage of 5-oxo-L-proline to form L-glutamate. The reaction is coupled to the hydrolysis of ATP to ADP and inorganic phosphate, so the energy of ATP drives the opening of the 5-oxo-L-proline ring. According to the NCBI Gene summary, the protein acts as a homodimer. Researchers studying this step of amino acid metabolism often work with an OPLAH antibody, an OPLAH ELISA kit or recombinant OPLAH protein to examine enzyme abundance and activity.
The OPLAH gene encodes 5-oxoprolinase, which UniProt assigns to the oxoprolinase family and to EC 3.5.2.9. The enzyme is found in the cytosol, within the cytoplasm. In HGNC the gene is classified in the group of microRNA protein coding host genes. Orthologues are annotated in both mouse and rat, each named Oplah, which allows the gene to be studied in common rodent models.
The enzyme is also known as OPLA and 5-Opase. Defects in the OPLAH gene are a cause of 5-oxoprolinase deficiency (OPLAHD), which links this enzyme to human disease. The recommended name, 5-oxoprolinase, describes its substrate, while the HGNC name, 5-oxoprolinase, ATP-hydrolysing, highlights the ATP-dependent nature of the reaction.
OPLAH converts 5-oxo-L-proline to L-glutamate, making it a direct subject of study in the metabolism of these two amino acid derivatives.
Defects in OPLAH cause 5-oxoprolinase deficiency (OPLAHD), so the gene and protein are relevant to research on this disorder.
The reaction couples ATP hydrolysis to ADP and inorganic phosphate, providing a model for studying energy-coupled hydrolysis by an oxoprolinase family member.
Mouse and rat both carry an Oplah orthologue, supporting comparative studies of 5-oxoprolinase function across species.
OPLAH, or 5-oxoprolinase, catalyses the cleavage of 5-oxo-L-proline to L-glutamate. The reaction is coupled to the hydrolysis of ATP to ADP and inorganic phosphate. It acts in the cytosol, and the NCBI Gene summary describes the active protein as a homodimer.
OPLAH is also written as OPLA, and the enzyme is called 5-Opase. UniProt uses the name 5-oxoprolinase, while HGNC gives the full name as 5-oxoprolinase, ATP-hydrolysing. The enzyme classification is EC 3.5.2.9, and the protein belongs to the oxoprolinase family.
Defects in the OPLAH gene are a cause of 5-oxoprolinase deficiency (OPLAHD). Mouse and rat each have an orthologue named Oplah. Cross-species use of any reagent depends on its own validation, so check the datasheet of any product for its validated reactivity.
Other metabolic enzymes and amino acid pathway targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O14841; product specifications from Assay Genie datasheets.