TRAM2
TRAM2 (translocation associated membrane protein 2, also known as KIAA0057) is a membrane component of the translocon at the endoplasmic reticulum. It is necessary for collagen type I synthesis and belongs to the TRAM family and the TLC domain containing gene group. Browse Assay Genie's current range of TRAM2 research reagents below, or read on for the biology behind the target.
TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane. The TRAM2 protein is necessary for collagen type I synthesis. It may couple the activity of the ER Ca(2+) pump SERCA2B with the activity of the translocon, which could raise the local Ca(2+) concentration at the site of collagen synthesis. A high Ca(2+) concentration may be needed for the molecular chaperones involved in collagen folding.
TRAM2 also has a role in membrane protein topology. It is required for proper insertion of the first transmembrane helix N-terminus of TM4SF20 into the ER lumen, and it may act as a ceramide sensor for regulated alternative translocation (RAT). The TRAM2 gene is located on chromosome 6 at 6p12.2 and encodes a protein that is classed as a membrane protein. The mouse ortholog is Tram2.
The protein is a member of the TRAM family and of the HGNC gene group of TLC domain containing proteins. It was previously catalogued under the symbol KIAA0057. The related protein TRAM1 belongs to the same translocon-associated group, which makes the two useful comparators when studying ER protein handling. Researchers working on collagen biosynthesis, ER calcium handling and translocation often study TRAM2 as a TRAM2 antibody target or by TRAM2 ELISA kit measurement.
TRAM2 is necessary for collagen type I synthesis, making it a focus for studies of how collagen is produced and folded in the ER.
TRAM2 may couple SERCA2B activity to the translocon, linking local Ca(2+) concentration to chaperone function.
As a translocon component, TRAM2 helps control the processing of nascent secretory and membrane proteins at the ER membrane.
TRAM2 is required for correct insertion of the TM4SF20 N-terminus into the ER lumen and may sense ceramide during RAT.
TRAM2 is a component of the ER translocon and is necessary for collagen type I synthesis. It may couple SERCA2B calcium pump activity with the translocon, raising local Ca(2+) for collagen-folding chaperones. It is also required for correct insertion of the TM4SF20 N-terminus into the ER lumen.
The HGNC name is translocation associated membrane protein 2, and UniProt lists translocating chain-associated membrane protein 2. KIAA0057 is a recorded alias. The protein belongs to the TRAM family, and its related family member TRAM1 is also studied in translocon biology.
A mouse ortholog, Tram2, is recorded for the human TRAM2 gene, so mouse models can be used to study its role. Source data supplied here do not list a rat ortholog. Check the datasheet of any product for its validated reactivity.
TRAM1, the other TRAM family member, and related translocon and ER proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q15035; product specifications from Assay Genie datasheets.