UBE2T
UBE2T (ubiquitin conjugating enzyme E2 T, also known as HSPC150 and FANCT) is a nuclear E2 ubiquitin-conjugating enzyme. It accepts ubiquitin from the E1 complex and catalyses its attachment to substrates, acting as the E2 for the Fanconi anaemia complex. Browse Assay Genie's current range of UBE2T research reagents below, or read on for the biology behind the target.
UBE2T is an E2 ubiquitin-conjugating enzyme. It accepts ubiquitin from the E1 complex and catalyses its covalent attachment to other proteins, and it is particularly associated with monoubiquitination. It is involved in mitomycin-C (MMC)-induced DNA repair. UBE2T acts as the specific E2 for the Fanconi anaemia complex: it associates with the E3 ubiquitin-protein ligase FANCL and catalyses monoubiquitination of FANCD2, a key step in the DNA damage pathway. It also mediates monoubiquitination of FANCL and FANCI.
UBE2T may contribute to the ubiquitination and degradation of BRCA1. In vitro, the enzyme can promote polyubiquitination using all seven ubiquitin lysine residues, but it may prefer Lys-11-, Lys-27-, Lys-48- and Lys-63-linked chains. The protein localises to the nucleus and belongs to the ubiquitin-conjugating enzyme family. In the HGNC classification it sits in both the ubiquitin conjugating enzymes E2 group and the Fanconi anaemia complementation groups.
The UBE2T gene was previously described as HSPC150, and the protein as ubiquitin-conjugating enzyme E2T; the symbol FANCT reflects its role in disease. According to NCBI Gene, defects in the gene are associated with Fanconi anaemia of complementation group T. Two transcript variants encoding different isoforms have been reported. Researchers studying the UBE2T gene or UBE2T protein typically do so in the context of DNA repair and ubiquitin signalling.
UBE2T is the E2 partner of FANCL and catalyses FANCD2 monoubiquitination, a key step in the Fanconi anaemia DNA damage pathway.
UBE2T is involved in mitomycin-C-induced DNA repair, making it relevant to studies of how cells respond to DNA damage.
In vitro, UBE2T can use all seven ubiquitin lysines and may prefer Lys-11, Lys-27, Lys-48 and Lys-63 linkages.
UBE2T may contribute to the ubiquitination and degradation of BRCA1, linking this E2 enzyme to BRCA1 turnover.
UBE2T accepts ubiquitin from the E1 complex and catalyses its attachment to other proteins, mainly as monoubiquitination. With the E3 ligase FANCL it monoubiquitinates FANCD2 in the Fanconi anaemia DNA damage pathway, and it also modifies FANCL and FANCI.
UBE2T is also known as HSPC150 and FANCT, and was previously named ubiquitin-conjugating enzyme E2T. NCBI Gene reports two transcript variants encoding different isoforms. The enzyme is classed as EC 2.3.2.23 and belongs to the ubiquitin-conjugating enzyme family.
Defects in UBE2T are associated with Fanconi anaemia of complementation group T, which is why it carries the FANCT symbol. The mouse ortholog is Ube2t. Check the datasheet of any product for its validated reactivity.
Other ubiquitin-conjugating E2 enzymes and related ubiquitin pathway proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NPD8; product specifications from Assay Genie datasheets.