TAF5L
TAF5L (TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, also known as PAF65B) is a nuclear WD repeat protein and a component of the PCAF histone acetylase complex. It contributes to transcriptional regulation and to control of embryonic stem cell state. Browse Assay Genie's current range of TAF5L research reagents below, or read on for the biology behind the target.
TAF5L functions as a component of the PCAF complex, which is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered the human version of the yeast SAGA complex. Together with TAF6L, TAF5L acts as an epigenetic regulator essential for somatic reprogramming. It regulates target genes through H3K9ac deposition and MYC recruitment, which trigger the MYC regulatory network to orchestrate gene expression programmes that control embryonic stem cell state.
The TAF5L gene encodes a protein of the WD repeat TAF5 family, and HGNC places it in the WD repeat domain containing gene group. The protein localises to the nucleus. It is structurally similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. The mouse ortholog is Taf5l.
According to the NCBI Gene summary, the PCAF histone acetylase complex is composed of more than 20 polypeptides, some of which are TAFs, and is required for myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors to facilitate complex assembly and transcription initiation. The older names for the gene describe it as a TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, and PAF65B remains a common alias in the literature.
TAF5L is a component of the PCAF complex, which acetylates histones in a nucleosomal context, making it relevant to studies of chromatin modification.
With TAF6L, TAF5L acts as an epigenetic regulator essential for somatic reprogramming, so it is a focus for cell identity research.
TAF5L regulates target genes through H3K9ac deposition and MYC recruitment to control gene expression programmes defining embryonic stem cell state.
The PCAF histone acetylase complex containing TAF5L is required for myogenic transcription and differentiation.
TAF5L is a component of the PCAF histone acetylase complex, which efficiently acetylates histones in a nucleosomal context. With TAF6L it is an epigenetic regulator essential for somatic reprogramming, acting through H3K9ac deposition and MYC recruitment to control embryonic stem cell state.
TAF5L is also known as PAF65B. Its full HGNC name is TATA-box binding protein associated factor 5 like, and earlier names describe it as a TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor of 65 kDa. Alternatively spliced transcript variants encoding different isoforms have been reported.
TAF5L belongs to the WD repeat TAF5 family and is structurally similar to the histone-like TAF, TAF5. It is encoded by the TAF5L gene in human, and the mouse ortholog is Taf5l. Check the datasheet of any product for its validated reactivity.
Other TAF family members and transcription-associated factors with research reagents at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O75529; product specifications from Assay Genie datasheets.