NELFCD
NELFCD (negative elongation factor complex member C/D, also known as NELF-C, NELF-D, TH1L, TH1 and HSPC130) is an essential subunit of the NELF complex. This nuclear complex works with DSIF to repress transcriptional elongation by RNA polymerase II. Browse Assay Genie's current range of NELFCD research reagents below, or read on for the biology behind the target.
NELFCD is a component of the negative elongation factor (NELF) complex. According to the NCBI Gene summary, the NELF complex interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is described as an essential part of the NELF complex, so it is a useful subject for studies of promoter-proximal pausing and the control of transcription after initiation. Researchers working on this area may look for a NELFCD antibody or a NELFCD ELISA kit to study the protein in cells and tissues.
The human NELFCD gene encodes a nuclear protein that belongs to the NELF-D family. HGNC places it in two gene groups: armadillo like helical domain containing, and negative elongation factor complex members. Alternative translation initiation site usage produces two isoforms with different N-termini. The mouse ortholog is Nelfcd.
The protein has several names in the literature. NELF-C and NELF-D reflect its place in the complex, while TH1L and TH1 come from the earlier name TH1-like (Drosophila homolog). HSPC130 is a further alias. In a microbial infection context, UniProt notes that the NELF complex is involved in HIV-1 latency, possibly through recruitment of PCF11 to paused RNA polymerase II. This links NELFCD to work on viral gene silencing as well as basic transcription biology.
NELFCD is an essential part of the NELF complex, which works with DSIF to repress elongation by RNA polymerase II.
As a NELF subunit, NELFCD is relevant to studies of paused RNA polymerase II and the factors recruited to it.
The NELF complex is implicated in HIV-1 latency, possibly through recruitment of PCF11 to paused RNA polymerase II.
Alternative translation initiation yields two NELFCD isoforms with different N-termini, which is relevant to protein-level studies of expression.
NELFCD is an essential subunit of the NELF complex. The complex interacts with the DSIF complex to repress transcriptional elongation by RNA polymerase II. UniProt also reports that the NELF complex is involved in HIV-1 latency, possibly via recruitment of PCF11 to paused RNA polymerase II.
NELFCD is also called NELF-C, NELF-D, TH1L, TH1 and HSPC130. Earlier names include TH1-like (Drosophila homolog). Alternative translation initiation site usage gives rise to two isoforms that differ in their N-termini, so check which isoform a reagent recognises.
A mouse ortholog, Nelfcd, is recorded for the human gene. The protein belongs to the NELF-D family. Cross-species use of any reagent depends on its own characterisation, so check the datasheet of any product for its validated reactivity.
Other NELF complex subunits, DSIF components and transcription-elongation factors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8IXH7; product specifications from Assay Genie datasheets.