TRIB1
TRIB1 (tribbles pseudokinase 1, also known as C8FW, GIG2 and TRB1) is an adapter protein that works with the COP1 ubiquitin ligase to direct protein degradation. It also binds MAPK kinases and regulates MAP kinase activation, although it has no kinase activity. Browse Assay Genie's current range of TRIB1 research reagents below, or read on for the biology behind the target.
TRIB1 acts as an adapter in protein degradation. It interacts with the COP1 ubiquitin ligase and alters its substrate specificity, directing COP1 activity towards CEBPA. The TRIB1 protein binds the recognition sequence of CEBPA selectively. The COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. Through this COP1-dependent control of CEBPA expression, TRIB1 regulates myeloid cell differentiation, and its COP1-binding domain controls macrophage, eosinophil and neutrophil differentiation.
TRIB1 also interacts with MAPK kinases and regulates the activation of MAP kinases, yet it has no kinase activity. NCBI Gene annotates the TRIB1 gene product with MAP kinase kinase binding, protein kinase inhibitor activity and transcription regulator inhibitor activity. It is involved in negative regulation of signal transduction, negative regulation of smooth muscle cell proliferation and regulation of MAP kinase activity. The protein is located in the cytoplasm and nucleus.
TRIB1 belongs to the Tribbles subfamily of the CAMK Ser/Thr protein kinase family, within the protein kinase superfamily. HGNC classifies it in the pseudokinase group and the Tribbles pseudokinase family. Its earlier name, tribbles homolog 1 (Drosophila), reflects the family's founding fly gene, and C8FW, GIG2 and TRB1 are recorded aliases. The mouse ortholog is Trib1. Researchers studying TRIB1 often look at its relationship with the related family member TRIB3.
TRIB1 regulates macrophage, eosinophil and neutrophil differentiation by altering CEBPA expression in a COP1-dependent manner.
TRIB1 changes COP1 substrate specificity by directing the ligase towards CEBPA, making it a model for adapter-controlled degradation.
TRIB1 binds MAPK kinases and regulates MAP kinase activation without catalytic activity, a feature of pseudokinase biology.
NCBI Gene links TRIB1 to negative regulation of smooth muscle cell proliferation and of signal transduction.
TRIB1 is an adapter that binds the COP1 ubiquitin ligase and directs its activity towards CEBPA, so regulating myeloid cell differentiation. It also interacts with MAPK kinases and regulates MAP kinase activation, but it has no kinase activity of its own.
TRIB1 is the HGNC symbol for tribbles pseudokinase 1. UniProt names the protein Tribbles homolog 1, and recorded aliases are C8FW, GIG2 and TRB1. The earlier HGNC name was tribbles homolog 1 (Drosophila).
No. TRIB1 belongs to the Tribbles subfamily of the CAMK Ser/Thr protein kinase family but is a pseudokinase without kinase activity. A mouse ortholog, Trib1, is listed; check the datasheet of any product for its validated reactivity.
Another Tribbles pseudokinase family member with research products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96RU8; product specifications from Assay Genie datasheets.