PRPF8
PRPF8 (pre-mRNA processing factor 8, also known as PRPC8, Prp8, hPrp8, SNRNP220 and RP13) is a core spliceosomal protein. It acts as a scaffold that organises snRNAs and proteins at splice sites during pre-mRNA splicing. Browse Assay Genie's current range of PRPF8 research reagents below, or read on for the biology behind the target.
PRPF8 plays a central role in pre-mRNA splicing as a core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. It is present in both the predominant U2-type spliceosome and the minor U12-type spliceosome. The protein functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs, and it is required for assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. It also positions the U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates so that splicing can occur, and it interacts with both the 5' and the 3' splice site.
Splicing proceeds through two sequential transesterification steps, and PRPF8 is reported to be essential for the catalytic step II. The protein contains several WD repeats, which function in protein-protein interactions. UniProt places it in the nucleus, including nuclear speckles. HGNC classifies the PRPF8 gene within the JAMM/MPN+ metallopeptidase family and the U5 small nuclear ribonucleoprotein group, and it is also listed under the spliceosomal Bact, C and P complexes.
The protein shows sequence similarity to yeast Prp8, which is reflected in earlier gene names such as PRP8 pre-mRNA processing factor 8 homolog (yeast). The mouse ortholog is Prpf8. The PRPF8 gene is a candidate gene for autosomal dominant retinitis pigmentosa, which explains the RP13 alias, making it a frequent subject in studies linking core splicing machinery to inherited retinal disease.
PRPF8 is a scaffold required for U4/U6-U5 tri-snRNP assembly, making it a key subject for work on how the spliceosome is built.
It positions the U2, U5 and U6 snRNAs and contacts both the 5' and 3' splice sites on pre-mRNA.
PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa, linking core splicing factors to inherited retinal disease.
As a component of both U2- and U12-dependent spliceosomes, PRPF8 allows comparison of major and minor splicing pathways.
PRPF8 is a core component of precatalytic, catalytic and postcatalytic spliceosomes. It acts as a scaffold for ordered assembly of spliceosomal proteins and snRNAs, is required for U4/U6-U5 tri-snRNP assembly, and is essential for catalytic step II of pre-mRNA splicing.
The protein is also called PRPC8, Prp8, hPrp8, SNRNP220 and RP13. Earlier HGNC names described it as a PRP8 pre-mRNA processing factor 8 homolog from yeast. UniProt lists the protein name as Pre-mRNA-processing-splicing factor 8.
PRPF8 is a candidate gene for autosomal dominant retinitis pigmentosa, and the RP13 alias reflects this. It resembles yeast Prp8 in sequence, and the mouse ortholog is Prpf8. Check the datasheet of any product for its validated reactivity.
Other pre-mRNA processing factors and spliceosome-associated proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q6P2Q9; product specifications from Assay Genie datasheets.