IQGAP1
IQGAP1 (IQ motif containing GTPase activating protein 1, also known as p195, KIAA0051, SAR1 and HUMORFA01) is a multidomain scaffold protein that regulates the dynamics and assembly of the actin cytoskeleton. It links cytoskeletal components, adhesion molecules and signalling proteins to control cell morphology and motility. Browse Assay Genie's current range of IQGAP1 research reagents below, or read on for the biology behind the target.
IQGAP1 plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. It is recruited to the cell cortex through interaction with ILK, which allows it to cooperate with its effector DIAPH1 to stabilise microtubules locally and permit stable insertion of caveolae into the plasma membrane. IQGAP1 binds activated CDC42 but does not stimulate its GTPase activity, and it also associates with calmodulin.
The IQGAP1 gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules and with several signalling molecules to regulate cell morphology and motility. UniProt reports localisation to the cell membrane, including apical and basolateral regions, the cytoplasm and cell cortex, and the nucleus.
Beyond cytoskeletal control, IQGAP1 may promote neurite outgrowth and may contribute to cell cycle progression after DNA replication arrest. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. Despite its name and Ras-GAP-like domain, the annotation indicates it does not stimulate CDC42 GTPase activity. The related family member IQGAP2 is a useful comparator when studying this scaffold group.
IQGAP1 regulates the assembly and dynamics of the actin cytoskeleton, making it a central protein for studies of cytoskeletal organisation.
By interacting with cytoskeletal proteins, adhesion molecules and signalling molecules, IQGAP1 regulates cell morphology and motility.
IQGAP1 cooperates with ILK and DIAPH1 to stabilise microtubules locally and allow stable insertion of caveolae into the plasma membrane.
IQGAP1 is upregulated by gene amplification in two gastric cancer cell lines and may contribute to cell cycle progression after DNA replication arrest.
IQGAP1 regulates the dynamics and assembly of the actin cytoskeleton. It is recruited to the cell cortex by ILK, cooperates with DIAPH1 to stabilise microtubules, and binds activated CDC42 and calmodulin. It also interacts with adhesion molecules and signalling proteins to influence cell morphology and motility.
The HGNC name is IQ motif containing GTPase activating protein 1, and UniProt lists Ras GTPase-activating-like protein IQGAP1. Aliases and previous symbols include p195, KIAA0051, SAR1 and HUMORFA01. It belongs to the IQ motif containing GTPase activating protein family, which also includes IQGAP2.
A mouse ortholog, Iqgap1, is recorded in the data, so rodent models can be used to study it; check the datasheet of any product for its validated reactivity. In disease terms, the NCBI summary notes IQGAP1 upregulation by gene amplification in two gastric cancer cell lines.
Other IQGAP family members and related cytoskeletal scaffold targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P46940; product specifications from Assay Genie datasheets.