RAD18
RAD18 (RAD18 E3 ubiquitin protein ligase, also known as RNF73) is an E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. It partners with the E2 enzyme UBE2B to mono-ubiquitinate DNA-associated PCNA. Browse Assay Genie's current range of RAD18 research reagents below, or read on for the biology behind the target.
RAD18 is an E3 ubiquitin-protein ligase that acts in postreplication repair of UV-damaged DNA. Postreplication repair fills the gaps left in a daughter strand when damaged DNA is replicated. RAD18 associates with the E2 ubiquitin-conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex, which mediates mono-ubiquitination of DNA-associated PCNA on Lys-164. The protein also has single-stranded DNA binding activity, linking it directly to DNA at sites of replication stress.
The human RAD18 gene encodes a protein that belongs to the RAD18 family. It is classed by HGNC among RING finger E3 ubiquitin protein ligases and among zinc finger proteins of the UBZ4 CCHC type. UniProt reports localisation to the nucleus and to the centrosome, part of the cytoplasmic microtubule organising centre. Researchers studying the RAD18 protein often examine it alongside its E2 partner and PCNA modification.
The gene summary notes that the protein is highly similar to the S. cerevisiae DNA damage repair protein Rad18, hence the previous name RAD18 homolog (S. cerevisiae). In yeast, Rad18 works through its interaction with Rad6, an ubiquitin-conjugating enzyme required for post-replication repair. The human protein likewise interacts with the human homolog of Rad6 through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens, which underlines the importance of the RING domain for RAD18 function.
RAD18 mediates repair of UV-damaged DNA by gap-filling of a daughter strand during replication of damaged templates.
The UBE2B-RAD18 complex mono-ubiquitinates DNA-associated PCNA on Lys-164, making RAD18 a focus for studying this modification.
RAD18 pairs a conserved RING finger with an E2 enzyme, offering a model for how E3 ligases engage ubiquitin-conjugating partners.
Mutation of the RAD18 ring-finger motif causes defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
RAD18 is an E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. With the E2 enzyme UBE2B it forms a complex that mono-ubiquitinates DNA-associated PCNA on Lys-164. It also binds single-stranded DNA.
The gene symbol RAD18 has also appeared as RNF73, and the previous HGNC name was RAD18 homolog (S. cerevisiae). The current approved name is RAD18 E3 ubiquitin protein ligase, and UniProt lists the protein as E3 ubiquitin-protein ligase RAD18.
Yes. Human RAD18 is highly similar to S. cerevisiae Rad18, which works with the ubiquitin-conjugating enzyme Rad6 in post-replication repair. A mouse ortholog, Rad18, also exists. Check the datasheet of any product for its validated reactivity.
Other RAD-family and DNA repair proteins with products at Assay Genie, useful alongside RAD18 in DNA damage response research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NS91; product specifications from Assay Genie datasheets.