UBE2H
UBE2H (ubiquitin conjugating enzyme E2 H, also known as UBCH, UBC8 and GID3) is an E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the E1 complex and catalyses its covalent attachment to other proteins. It transfers ubiquitin to MAEA, a core subunit of the CTLH E3 ligase complex. Browse Assay Genie's current range of UBE2H research reagents below, or read on for the biology behind the target.
Modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H acts at the middle step. It accepts ubiquitin from the E1 complex and catalyses its covalent attachment to other proteins.
A specific substrate of this E2 is MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex, so UBE2H transfers ubiquitin to a subunit of an E3 assembly. In vitro, the enzyme catalyses 'Lys-11'- and 'Lys-48'-linked polyubiquitination, and it is able to ubiquitinate histone H2A. These activities are described from in vitro work, and the UBE2H protein is classed in the ubiquitin-conjugating enzyme family with EC number 2.3.2.23.
The UBE2H gene belongs to the HGNC group of ubiquitin conjugating enzymes E2. Its previous name was ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast), and the aliases UBCH, UBC8 and GID3 are still in use. According to RefSeq, the encoded protein sequence is 100% identical to the mouse homolog (Ube2h) and 98% identical to the frog and zebrafish homologs, indicating strong conservation. Three alternatively spliced transcript variants have been found for the gene, and they encode distinct isoforms.
UBE2H is an E2 enzyme in the E1-E2-E3 cascade that tags abnormal or short-lived proteins for degradation.
UBE2H transfers ubiquitin to MAEA, a core component of the CTLH complex, making it relevant to studies of this E3 ligase.
In vitro, UBE2H catalyses Lys-11- and Lys-48-linked polyubiquitination, which makes it useful for studying chain-type specificity.
UBE2H can ubiquitinate histone H2A in vitro, so it features in biochemical work on histone modification.
UBE2H is an E2 ubiquitin-conjugating enzyme. It accepts ubiquitin from the E1 complex and catalyses its covalent attachment to other proteins, including MAEA, a core component of the CTLH E3 ligase complex. In vitro it catalyses Lys-11- and Lys-48-linked polyubiquitination.
UBE2H is also known as UBCH, UBC8 and GID3, and was formerly named ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast). RefSeq reports three alternatively spliced transcript variants for the gene, which encode distinct isoforms of the protein.
Yes. According to RefSeq, the human UBE2H protein sequence is 100% identical to the mouse homolog (Ube2h) and 98% identical to the frog and zebrafish homologs. Check the datasheet of any product for its validated reactivity.
Other ubiquitin-conjugating E2 enzymes and related ubiquitin ligases with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P62256; product specifications from Assay Genie datasheets.