P4HA3
P4HA3 (prolyl 4-hydroxylase subunit alpha 3, also written C-P4Halpha(III)) is an alpha subunit of prolyl 4-hydroxylase, an enzyme central to collagen synthesis. It catalyses the formation of 4-hydroxyproline in collagens and other proteins. Browse Assay Genie's current range of P4HA3 research reagents below, or read on for the biology behind the target.
P4HA3 catalyses the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This hydroxylation is essential for the proper three-dimensional folding of newly synthesised procollagen chains. The P4HA3 protein is one of several types of alpha subunit of prolyl 4-hydroxylase and provides the major part of the catalytic site of the active enzyme.
Prolyl 4-hydroxylase is a key enzyme in collagen synthesis, composed of two identical alpha subunits and two beta subunits. P4HA3 is found in the lumen of the endoplasmic reticulum, where procollagen chains are processed. The protein belongs to the P4HA family and is classified by HGNC within the iron (II) and 2-oxoglutarate dependent oxygenases, in the prolyl 4-hydroxylase subunits group, with the enzyme classified as EC 1.14.11.2. Alternative splicing of the P4HA3 gene results in multiple transcript variants.
The gene has previously been named procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III, and prolyl 4-hydroxylase, alpha polypeptide III. Mouse and rat both carry an orthologous P4ha3 gene, so the enzyme can be studied in common model organisms. Researchers working on collagen biosynthesis and procollagen folding study P4HA3 alongside the other alpha subunit types that can form the active enzyme.
P4HA3 forms part of the prolyl 4-hydroxylase that hydroxylates proline residues, a key step in collagen synthesis.
4-hydroxyproline formation is essential for correct three-dimensional folding of newly synthesised procollagen chains, making P4HA3 relevant to folding studies.
As an iron (II) and 2-oxoglutarate dependent oxygenase, P4HA3 is a model for studying catalytic subunits of hydroxylase enzymes.
P4HA3 acts in the endoplasmic reticulum lumen, supporting work on post-translational modification of collagens and other proteins.
P4HA3 catalyses the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. As an alpha subunit of prolyl 4-hydroxylase, it provides the major part of the catalytic site, and the modification is essential for procollagen folding.
P4HA3 is also written C-P4Halpha(III). Older names include prolyl 4-hydroxylase, alpha polypeptide III. Alternative splicing of the gene results in multiple transcript variants. It is one of several types of prolyl 4-hydroxylase alpha subunit, and belongs to the P4HA family.
Yes. Mouse and rat each have an ortholog named P4ha3, so the enzyme can be studied in rodent models. Check the datasheet of any product for its validated reactivity before use in a given species.
Other prolyl 4-hydroxylase subunits, collagen proteins and related collagen-synthesis targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q7Z4N8; product specifications from Assay Genie datasheets.