PSAT1
PSAT1 (phosphoserine aminotransferase 1, also known as PSA or PSAT) is a pyridoxal 5'-phosphate-dependent aminotransferase that catalyses the second step of the phosphorylated pathway of L-serine biosynthesis. It also acts as an inhibitor of ferroptosis in response to interferon-gamma. Browse Assay Genie's current range of PSAT1 research reagents below, or read on for the biology behind the target.
PSAT1 is involved in L-serine biosynthesis through the phosphorylated pathway, a three-step route that converts the glycolytic intermediate 3-phospho-D-glycerate into L-serine. PSAT1 catalyses the second step: the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.
The enzyme has a second role in cell death control. PSAT1 acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting the stability of GPX4. Following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, which leads to GPX4 hydroxylation and stability.
The PSAT1 gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family, SerC subfamily, and HGNC places it in the transaminases gene group. Alternative splicing produces multiple transcript variants, and pseudogenes have been defined on chromosomes 1, 3 and 8. Mutations in the gene are associated with phosphoserine aminotransferase deficiency, and decreased expression may be associated with schizophrenia. The mouse ortholog is Psat1, which makes the mouse a natural system for studying the enzyme. Researchers studying serine metabolism or ferroptosis may look for a PSAT1 antibody, PSAT1 ELISA kit or recombinant PSAT1 protein to investigate this enzyme.
PSAT1 catalyses the second step of the phosphorylated pathway that converts 3-phospho-D-glycerate into L-serine.
PSAT1 inhibits ferroptosis in response to interferon-gamma by promoting GPX4 stability through EGLN3-mediated hydroxylation.
Mutations in the PSAT1 gene are associated with phosphoserine aminotransferase deficiency.
Decreased PSAT1 expression may be associated with schizophrenia, according to the NCBI Gene summary.
PSAT1 catalyses the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine and alpha-ketoglutarate, the second step of L-serine biosynthesis. It also inhibits ferroptosis in response to interferon-gamma by stabilising GPX4.
PSAT1 is also known as PSA and PSAT, and its full name is phosphoserine aminotransferase 1. UniProt lists the protein as phosphoserine aminotransferase. Alternative splicing of the gene results in multiple transcript variants.
Mutations in the PSAT1 gene are associated with phosphoserine aminotransferase deficiency, and decreased expression may be associated with schizophrenia. The mouse ortholog is Psat1. Check the datasheet of any product for its validated reactivity.
Serine biosynthesis enzymes, ferroptosis regulators and pathway partners that may also be of interest.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y617; product specifications from Assay Genie datasheets.