USP13
USP13 (ubiquitin specific peptidase 13, also known as IsoT-3) is a cytoplasmic deubiquitinase that removes ubiquitin from target proteins including BECN1, SKP2, MITF and USP10. Through these substrates it influences autophagy, ERAD, cell cycle progression and the DNA damage response. Browse Assay Genie's current range of USP13 research reagents below, or read on for the biology behind the target.
USP13 is a deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10. In autophagy, it deubiquitinates BECN1, a key regulator of the pathway, which stabilises PIK3C3/VPS34-containing complexes. USP13 can also form a deubiquitination complex with NEDD4 that stabilises VPS34 and promotes autophagy. It deubiquitinates USP10, an essential regulator of p53/TP53 stability, and PIK3C3/VPS34-containing complexes in turn regulate USP13 stability. This suggests a regulatory loop in which these complexes control p53/TP53 levels via USP10 and USP13.
USP13 also acts in protein quality control and cell cycle control. Recruited by nuclear UFD1, it deubiquitinates SKP2 and so regulates endoplasmic reticulum-associated degradation (ERAD). It further influences ERAD by deubiquitinating UBL4A, a component of the BAG6/BAT3 complex. It stabilises cell cycle proteins such as SKP2 and AURKB, and helps maintain genomic stability and DNA replication checkpoint activation through RAP80 and TOPBP1. It stabilises SIAH2 independently of its catalytic activity, by binding ubiquitinated SIAH2 and impairing its autoubiquitination.
USP13 deubiquitinates HMGB1, driving its nucleocytoplasmic localisation and secretion. It has opposing roles in innate immunity: it positively regulates type I and type II interferon signalling by deubiquitinating STAT1, but negatively regulates the antiviral response by deubiquitinating STING1. The USP13 protein belongs to the peptidase C19 family and is classed by HGNC among the ubiquitin specific peptidases. Its former name was ubiquitin specific protease 13 (isopeptidase T-3). NCBI Gene annotates BAT3 complex binding, peptidase activity and proteasome binding, with predicted localisation to the nucleoplasm, cytosol and nucleus. The mouse ortholog is Usp13.
USP13 deubiquitinates BECN1 and stabilises PIK3C3/VPS34-containing complexes, making it a point of control in autophagy.
USP13 acts on SKP2 and UBL4A to regulate endoplasmic reticulum-associated degradation and the BAG6/BAT3 complex.
USP13 stabilises SKP2 and AURKB and regulates RAP80 and TOPBP1, linking it to checkpoint activation and the DNA damage response.
USP13 deubiquitinates STAT1 and STING1, with opposing effects on interferon signalling and the antiviral response.
USP13 is a deubiquitinase that removes ubiquitin from substrates including BECN1, SKP2, USP10, UBL4A, HMGB1, STAT1 and STING1. Through these targets it regulates autophagy, ERAD, cell cycle progression, the DNA damage response and interferon signalling.
USP13 is the HGNC symbol for ubiquitin specific peptidase 13. UniProt names the protein ubiquitin carboxyl-terminal hydrolase 13, and IsoT-3 is a listed alias. The previous gene name was ubiquitin specific protease 13 (isopeptidase T-3). Its enzyme classification is EC 3.4.19.12.
A mouse ortholog, Usp13, is listed for the human gene. The source data give no rat ortholog. Check the datasheet of any product for its validated reactivity before choosing a reagent for a given species.
Other ubiquitin specific peptidases, including USP10, a substrate of USP13, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q92995; product specifications from Assay Genie datasheets.