UBE2F
UBE2F, also known as NCE2, is a NEDD8-conjugating enzyme of the E2 class that is involved in protein neddylation. It belongs to the ubiquitin-conjugating enzyme family, UBE2F subfamily, and the HGNC lists it among the ubiquitin conjugating enzymes E2. Browse Assay Genie's current range of UBE2F research reagents below, or read on for the biology behind the target.
UBE2F is an E2 enzyme that enables NEDD8 conjugating enzyme activity and is involved in protein neddylation, the covalent attachment of the ubiquitin-like modifier NEDD8 to target proteins. It is classified under EC 2.3.2.34. One documented role concerns cullin-5 (CUL5): UBE2F mediates neddylation of CUL5, a step required in the cullin-RING ligase pathway used by HIV-1.
In cells infected with HIV-1, UBE2F participates in ubiquitination and degradation of APOBEC3G that is mediated by the viral Vif protein. It does so by neddylating CUL5. This makes the UBE2F protein a point of interest for researchers studying how viral accessory proteins exploit host ubiquitin-like modification machinery. Predicted localisation of the enzyme is in the cytosol and nucleus.
The UBE2F gene is the official HGNC symbol for ubiquitin conjugating enzyme E2 F (putative), and the previous name was ubiquitin-conjugating enzyme E2F (putative). NCE2 is an alias. UniProt places the protein in the ubiquitin-conjugating enzyme family, UBE2F subfamily, and the HGNC groups it with the ubiquitin conjugating enzymes E2. Orthologues are annotated in mouse and rat, both named Ube2f.
UBE2F is a NEDD8-conjugating enzyme involved in protein neddylation, so it is a relevant target for studying how NEDD8 is transferred to substrates.
UBE2F mediates neddylation of cullin-5 (CUL5), linking this E2 enzyme to the regulation of cullin-based ubiquitination pathways.
During HIV-1 infection, UBE2F participates in Vif-mediated ubiquitination and degradation of APOBEC3G through CUL5 neddylation.
As a member of the UBE2F subfamily of ubiquitin-conjugating enzymes, UBE2F is useful for comparing E2 specificity across ubiquitin and ubiquitin-like pathways.
UBE2F is an E2 enzyme with NEDD8 conjugating activity and is involved in protein neddylation. In HIV-1 infection it neddylates cullin-5 (CUL5), which participates in Vif-mediated ubiquitination and degradation of APOBEC3G. Its predicted localisation is the cytosol and nucleus.
The HGNC name is ubiquitin conjugating enzyme E2 F (putative), and the UniProt protein name is NEDD8-conjugating enzyme UBE2F. NCE2 is a listed alias. The earlier HGNC name was ubiquitin-conjugating enzyme E2F (putative). The sources provided do not describe isoforms.
Orthologues of UBE2F are annotated in mouse and rat, both named Ube2f. The human protein belongs to the ubiquitin-conjugating enzyme family, UBE2F subfamily. Researchers working with rodent samples should check the datasheet of any product for its validated reactivity.
Other ubiquitin-conjugating E2 enzymes and related ubiquitin ligases that act in ubiquitin and ubiquitin-like modification pathways.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q969M7; product specifications from Assay Genie datasheets.