MEIS2
MEIS2 (Meis homeobox 2, also known as MRG1 or HsT18361) is a TALE-class homeodomain transcription regulator. It partners with HOX and PBX proteins to shape DNA-binding complexes that control gene expression during development. Browse Assay Genie's current range of MEIS2 research reagents below, or read on for the biology behind the target.
MEIS2 is involved in transcriptional regulation. It binds to HOX or PBX proteins to form dimers, or binds a DNA-bound dimer of PBX and HOX proteins, and is thought to help stabilise the homeoprotein-DNA complex. In pancreatic acinar cells, isoform 3 is required for the activity of a PDX1:PBX1b:MEIS2b complex that activates the ELA1 enhancer. That complex binds the enhancer B element and cooperates with the PTF1 complex on enhancer A, although MEIS2 itself is not involved in complex DNA-binding. MEIS2, probably with PBX1, also contributes to transcriptional regulation by KLF4.
MEIS2 can also act directly on promoters. Isoforms 3 and 4 bind an EPHA8 promoter sequence containing the motif 5'-CGGTCA-3'. With a PBX protein such as PBX2, this is proposed to drive EPHA8 activation in the developing midbrain. The protein can bind 5'-TGACAG-3' in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription, whereas isoform 5 cannot. MEIS2 may also be involved in regulating myeloid differentiation. It localises to the nucleus and to the perinuclear cytoplasm.
The MEIS2 gene encodes a member of the TALE ('three amino acid loop extension') family of homeodomain proteins, which UniProt places in the TALE/MEIS homeobox family. TALE homeobox proteins are highly conserved transcription regulators, and several members are essential contributors to developmental programmes. Multiple transcript variants encode distinct isoforms. HGNC lists the gene within the TALE class homeoboxes and among microRNA protein coding host genes. Earlier names include Meis (mouse) homolog 2 and Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse). The mouse ortholog is Meis2, and the related paralogue MEIS1 is also studied.
TALE homeobox proteins are conserved regulators, and several members are essential contributors to developmental programmes.
MEIS2 binds HOX or PBX proteins and is thought to stabilise homeoprotein-DNA complexes, making it a model for cooperative DNA binding.
MEIS2 is proposed to help activate EPHA8 in the developing midbrain and can activate DRD1 transcription.
Isoform 3 supports an enhancer complex at ELA1 in pancreatic acinar cells, and MEIS2 may be involved in myeloid differentiation.
MEIS2 is a transcriptional regulator. It binds HOX or PBX proteins, or a DNA-bound PBX-HOX dimer, and is thought to stabilise the homeoprotein-DNA complex. It also contributes to activation of targets such as ELA1, EPHA8 and DRD1, and may be involved in myeloid differentiation.
MEIS2 is also called MRG1 and HsT18361, and earlier names include Meis (mouse) homolog 2. Multiple transcript variants encode distinct isoforms. Isoforms differ in function: isoforms 3 and 4 bind the EPHA8 promoter motif, while isoform 5 cannot activate DRD1 transcription.
MEIS2 belongs to the TALE/MEIS homeobox family, and TALE homeobox proteins are highly conserved transcription regulators. A mouse ortholog, Meis2, exists. MEIS1 is a related family member. Check the datasheet of any product for its validated reactivity.
Related TALE/MEIS homeobox family members and transcription factors that partner with MEIS2.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O14770; product specifications from Assay Genie datasheets.