MAN2B1
MAN2B1 (mannosidase alpha class 2B member 1, also known as LAMAN and MANB) is the lysosomal alpha-mannosidase, a glycosyl hydrolase that removes terminal alpha-linked mannose from oligosaccharides produced during the breakdown of N-linked glycoproteins. Browse Assay Genie's current range of MAN2B1 research reagents below, or read on for the biology behind the target.
MAN2B1 encodes a lysosomal alpha-mannosidase that hydrolyses terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. It can act on a variety of glycan substrates containing terminal alpha-mannosidic linkages, cleaving alpha 1,2-, alpha 1,3- and alpha 1,6-linked mannose on oligosaccharides generated by N-glycoprotein degradation pathways. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. UniProt lists the protein in the lysosome lumen and as secreted.
The MAN2B1 gene product belongs to the glycosyl hydrolase 38 family and sits within the HGNC gene group of alpha class 2 mannosidases. Maturation is stepwise. The full-length protein first loses a 49 amino acid leader sequence, and the remainder is processed into three peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). The 70 kDa peptide is then cleaved further into peptides A, B and C, which remain disulfide-linked. Alternatively spliced transcript variants encoding different isoforms have been reported for the gene.
Defects in the MAN2B1 gene are associated with lysosomal alpha-mannosidosis, which makes the enzyme a reference point for work on lysosomal glycan catabolism. The previous HGNC name was mannosidase, alpha, class 2B, member 1, and the mouse ortholog is Man2b1. Researchers studying MAN2B1 antibody, MAN2B1 ELISA kit or MAN2B1 protein reagents typically work on glycoprotein turnover, lysosomal storage and enzyme processing.
Defects in MAN2B1 are associated with lysosomal alpha-mannosidosis, making it central to studies of this storage disorder.
MAN2B1 is needed for catabolism of N-linked carbohydrates released during glycoprotein turnover, so it informs glycan degradation research.
It cleaves alpha 1,2-, 1,3- and 1,6-linked mannose, a useful model for linkage specificity in glycosyl hydrolase family 38.
Multi-step proteolytic maturation into disulfide-linked peptides makes MAN2B1 a case study in lysosomal enzyme processing.
MAN2B1 is a lysosomal alpha-mannosidase that hydrolyses terminal alpha-D-mannose residues. It cleaves alpha 1,2-, alpha 1,3- and alpha 1,6-linked mannose on oligosaccharides generated by N-glycoprotein degradation, and is necessary for catabolism of N-linked carbohydrates during glycoprotein turnover.
MAN2B1 is also known as LAMAN and MANB, and UniProt names the protein lysosomal alpha-mannosidase. Its earlier HGNC name was mannosidase, alpha, class 2B, member 1. Alternatively spliced transcript variants encoding different isoforms have been found, and the mature enzyme is processed into several peptides.
Defects in the MAN2B1 gene are associated with lysosomal alpha-mannosidosis. A mouse ortholog, Man2b1, exists, so the enzyme can be studied in mouse models; check the datasheet of any product for its validated reactivity.
Other mannosidases and mannose-related proteins that sit alongside MAN2B1 in glycan processing and lysosomal biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O00754; product specifications from Assay Genie datasheets.