CBY1
CBY1 (chibby 1, beta catenin antagonist) is a small member of the chibby family that inhibits Wnt/Wingless signalling by binding beta-catenin. It is also known as Chibby, Cby, PIGEA-14 and C22orf2. Browse Assay Genie's current range of CBY1 research reagents below, or read on for the biology behind the target.
CBY1 inhibits the Wnt/Wingless pathway by binding directly to CTNNB1/beta-catenin, interacting with its C-terminal region. In doing so it blocks beta-catenin-mediated transcriptional activation, competing with TCF/LEF transcription factors for access to beta-catenin. Because beta-catenin is a transcriptional activator and an oncoprotein implicated in the development of several cancers, this antagonism makes the CBY1 protein a useful subject for studies of Wnt signalling and its control.
Beyond Wnt regulation, CBY1 has been shown to take part in the intracellular trafficking of polycystin-2/PKD2, and possibly of other intracellular proteins. It also promotes adipocyte and cardiomyocyte differentiation. Consistent with these roles, UniProt records a broad subcellular distribution: nuclear speckles and nucleus, cytoplasm, the Golgi apparatus and trans-Golgi network, and the centrosome and centriole. It is also found at the basal body of the cilium and in the flagellum. The protein belongs to the chibby family.
The CBY1 gene has had several names. Earlier designations include chromosome 22 open reading frame 2 (C22orf2), PKD2 interactor, golgi and endoplasmic reticulum associated 1 (PIGEA14, PGEA1), and chibby homolog 1 (Drosophila). Two transcript variants encoding different isoforms have been reported. Mouse and rat both carry an ortholog named Cby1, which allows comparative work across model systems.
CBY1 competes with TCF/LEF factors for beta-catenin, so it is a direct tool for studying how transcriptional activation by beta-catenin is restrained.
Beta-catenin is an oncoprotein involved in several cancers, and CBY1 inhibits its oncogenic transcriptional activity through direct binding.
CBY1 promotes adipocyte and cardiomyocyte differentiation, making it relevant to studies of lineage commitment in these cell types.
CBY1 regulates trafficking of polycystin-2/PKD2 and localises to the Golgi, centrioles and ciliary basal body, linking it to trafficking and cilium research.
CBY1 inhibits Wnt/Wingless signalling by binding beta-catenin and competing with TCF/LEF transcription factors, which reduces beta-catenin-driven transcription. It also regulates trafficking of polycystin-2/PKD2 and promotes adipocyte and cardiomyocyte differentiation.
CBY1 is also called Chibby, Cby, Chibby1, PIGEA14, PIGEA-14, PGEA1 and C22orf2. Older names include PKD2 interactor, golgi and endoplasmic reticulum associated 1, and chibby homolog 1 (Drosophila). Two transcript variants encoding different isoforms have been described.
Yes. Mouse and rat each have an ortholog named Cby1, so the protein can be studied in common model organisms. Check the datasheet of any product for its validated reactivity.
Beta-catenin, polycystin-2 and TCF/LEF transcription factors that connect CBY1 to Wnt signalling and trafficking research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y3M2; product specifications from Assay Genie datasheets.