UBE2B
UBE2B (ubiquitin conjugating enzyme E2 B) is an E2 enzyme that accepts ubiquitin from the E1 activating enzyme and passes it to an E3 ligase. It is also known as UBC2, HHR6B and RAD6B, and it contributes to histone modification and DNA damage tolerance. Browse Assay Genie's current range of UBE2B research reagents below, or read on for the biology behind the target.
UBE2B is an E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to an E3 ubiquitin-protein ligase. In vitro it catalyses 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. With the E3 enzyme BRE1 (RNF20 and/or RNF40), it monoubiquitinates histone H2B at 'Lys-120' to form H2BK120ub1. This mark tags chromatin for transcriptional activation, elongation by RNA polymerase II and telomeric silencing, and is a prerequisite for H3K4me and H3K79me formation.
UBE2B also works in genome maintenance and protein turnover. It associates with the E3 ligase RAD18 to form the UBE2B-RAD18 complex, which monoubiquitinates DNA-associated PCNA at 'Lys-164'. With the E3 enzyme UBR4, it takes part in N-end rule-dependent protein degradation. It may also play a role in DNA repair and in neurite outgrowth. The UBE2B protein is assigned to the ubiquitin-conjugating enzyme family and to the HGNC group of ubiquitin conjugating enzymes E2. UniProt lists its locations as the cell membrane and nucleus.
Ubiquitination targets abnormal or short-lived proteins for degradation and involves three enzyme classes: E1 activating enzymes, E2 conjugating enzymes and E3 ligases. The UBE2B gene encodes an E2 enzyme that RefSeq describes as required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat and rabbit homologs, so it is highly conserved in eukaryotic evolution. Earlier names include ubiquitin-conjugating enzyme E2B (RAD6 homolog), reflecting its relationship to yeast RAD6; the aliases RAD6B and HHR6B reflect the same history.
UBE2B helps form H2BK120ub1, a histone mark linked to transcriptional activation, elongation by RNA polymerase II, telomeric silencing and H3K4me and H3K79me formation.
With RAD18, UBE2B monoubiquitinates DNA-associated PCNA at Lys-164, and RefSeq describes the enzyme as required for post-replicative DNA damage repair.
In vitro, UBE2B catalyses Lys-11-, Lys-48- and Lys-63-linked polyubiquitination, making it useful for studying E2 and E3 pairing and chain specificity.
UBE2B acts with the E3 enzyme UBR4 in N-end rule-dependent protein degradation, and it may also be involved in neurite outgrowth.
UBE2B is an E2 enzyme that takes ubiquitin from E1 and transfers it to an E3 ligase. With RNF20/RNF40 it monoubiquitinates histone H2B at Lys-120, and with RAD18 it monoubiquitinates PCNA at Lys-164. It also works with UBR4 in N-end rule degradation.
UBE2B is also known as UBC2, HHR6B and RAD6B. Earlier names include ubiquitin-conjugating enzyme E2B (RAD6 homolog). UniProt calls the protein ubiquitin-conjugating enzyme E2 B, and its EC number is 2.3.2.23. The human gene is HGNC:12473.
Very highly. NCBI reports that the human UBE2B protein sequence is 100% identical to the mouse, rat and rabbit homologs, indicating strong conservation in eukaryotic evolution. The mouse and rat orthologs are both named Ube2b. Check the datasheet of any product for its validated reactivity.
Other ubiquitin-conjugating E2 enzymes, related E3 and E4 ligases and ubiquitin pathway targets.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P63146; product specifications from Assay Genie datasheets.