Anti-UBE2B Antibody (CAB6315)

Antibody Name:	Anti-UBE2B Antibody
Antibody SKU:	CAB6315
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, Jurkat, NIH/3T3, Mouse heart, Mouse brain, Mouse skeletal muscle, Rat heart, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTP ARRR LMRD FKRL QEDP PVGV SGAP SENN IMQW NAVI FGPE GTPF EDGT FKLV IEFS EEYP NKPP TVRF LSKM FHPN VYAD GSIC LDIL QNRW SPTY DVSS ILTS IQSL LDEP NPNS PANS QAAQ LYQE NKRE YEKR VSAI VEQS WNDS
Gene ID:	7320
Uniprot:	P63146
Cellular Location:	Cell membrane, Nucleus
Calculated MW:	17kDa
Observed MW:	16kDa

Synonyms:

UBE2B, E2-17kDa, HHR6B, HR6B, RAD6B, UBC2

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

UniProt Protein Function:	UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.
UniProt Protein Details:	Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: XY body; nuclear chromatin; cytoplasm; plasma membrane; replication fork; chromatin; nucleus Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: response to drug; protein monoubiquitination; ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; maintenance of chromatin silencing; response to hypoxia; sperm axoneme assembly; spermatogenesis; chiasma formation; response to DNA damage stimulus; histone H2A ubiquitination; response to UV; negative regulation of apoptosis
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
UniProt Code:	P63146
NCBI GenInfo Identifier:	52783814
NCBI Gene ID:	7320
NCBI Accession:	P63146.1
UniProt Related Accession:	P63146
Molecular Weight:
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 B
NCBI Synonym Full Names:	ubiquitin conjugating enzyme E2 B
NCBI Official Symbol:	UBE2B
NCBI Official Synonym Symbols:	HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 B
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 B
UniProt Synonym Protein Names:	RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2B
UniProt Entry Name:	UBE2B_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2B antibody (CAB6315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using UBE2B Antibody (CAB6315) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using UBE2B Antibody (CAB6315) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
UBE2B Antibody (PACO13021)	Human Ubiquitin-conjugating enzyme E2 B (UBE2B) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)