TRAF2
TRAF2 (TNF receptor-associated factor 2, also known as TRAP3 and RNF117) is a RING-type E3 ubiquitin-protein ligase that transmits signals from TNF receptor superfamily members. It regulates NF-kappa-B and JNK activation and plays a central role in cell survival and apoptosis. Browse Assay Genie's current range of TRAF2 research reagents below, or read on for the biology behind the target.
TRAF2 is an E3 ubiquitin-protein ligase that regulates activation of NF-kappa-B and JNK and has a central role in controlling cell survival and apoptosis. It catalyses 'Lys-63'-linked ubiquitination of target proteins including BIRC3, IKBKE, MLST8, RIPK1 and TICAM1. It is also an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes ubiquitination by bringing substrates into contact with other E3 ligases. It further contributes to NF-kappa-B activation mediated by EIF2AK2/PKR.
As a member of the TNF receptor-associated factor family, TRAF2 associates with and mediates signal transduction from members of the TNF receptor superfamily. It interacts directly with TNF receptors and forms a heterodimeric complex with TRAF1, and it is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The TRAF2-TRAF1 complex interacts with inhibitor-of-apoptosis proteins (IAPs) and mediates anti-apoptotic signals from TNF receptors. Its interaction with TRADD ensures recruitment of IAPs, which directly inhibit caspase activation. The TRAF2 gene is also classed by HGNC among ring finger proteins, TRAF-type zinc fingers and microRNA protein coding host genes.
TRAF2 also regulates other ubiquitin and signalling components. It controls BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and degradation, independently of its RING-type zinc finger domain. Conversely, BIRC2/c-IAP1 can ubiquitinate TRAF2 and induce its degradation, potentiating TNF-induced apoptosis. TRAF2 additionally regulates assembly of mTORC1 and mTORC2 by mediating Lys-63-linked ubiquitination of MLST8, which inhibits mTORC2 formation and facilitates mTORC1 assembly. It is required for normal antibody isotype switching from IgM to IgG. Multiple alternatively spliced transcript variants have been reported, though the biological validity of only one has been determined.
TRAF2 transduces signals from TNF receptor superfamily members and is required for TNF-alpha-mediated activation of JNK and NF-kappaB.
With TRAF1, TRADD and IAPs, TRAF2 mediates anti-apoptotic signals and supports inhibition of caspase activation.
TRAF2 catalyses Lys-63-linked ubiquitination of substrates such as RIPK1, IKBKE and TICAM1 and assembles E3 ligase complexes.
TRAF2 ubiquitinates MLST8, inhibiting mTORC2 formation while facilitating mTORC1 assembly, linking ubiquitin signalling to mTOR biology.
TRAF2 is an E3 ubiquitin-protein ligase that regulates NF-kappa-B and JNK activation and plays a central role in cell survival and apoptosis. It catalyses Lys-63-linked ubiquitination of targets such as RIPK1 and MLST8, and mediates anti-apoptotic signals from TNF receptors.
TRAF2 is the HGNC symbol for TNF receptor associated factor 2. Aliases include TRAP3 and RNF117, the latter reflecting its RING finger. UniProt lists the protein as TNF receptor-associated factor 2. Several alternatively spliced transcript variants exist, but only one has been shown to be biologically valid.
TRAF2 has a mouse ortholog, Traf2. It belongs to the TNF receptor-associated factor family, alongside TRAF3, TRAF4, TRAF5, TRAF6 and TRAF7, and forms a heterodimeric complex with TRAF1. Check the datasheet of any product for its validated reactivity.
Other members of the TNF receptor-associated factor family, which transduce signals from TNF receptor superfamily members.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q12933; product specifications from Assay Genie datasheets.