SRP54
SRP54 (signal recognition particle 54) is the GTP-binding subunit of the signal recognition particle, a ribonucleoprotein complex that directs secretory and membrane proteins to the endoplasmic reticulum. It binds signal sequences as they emerge from the ribosome and carries GTPase activity. Browse Assay Genie's current range of SRP54 research reagents below, or read on for the biology behind the target.
SRP54 is a component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The protein binds to the signal sequence of presecretory proteins when they emerge from the ribosome. As part of the SRP, it associates with the SRP receptor (SR) component SRPRA to bring these proteins to the ER membrane.
Mechanistically, SRP54 displays basal GTPase activity and stimulates reciprocal GTPase activation of the SR subunit SRPRA. The two proteins form a guanosine 5'-triphosphate (GTP)-dependent complex, and SR compaction together with GTPase-mediated rearrangement of SR drives SRP-mediated cotranslational protein translocation into the ER. SRP54 requires SRP9/SRP14 and/or SRP19 to interact stably with RNA, and it is reported to bind 7S RNA. It belongs to the GTP-binding SRP family, SRP54 subfamily, and the SRP54 gene sits in the HGNC groups for the signal recognition particle and its GTPases. UniProt places the protein in the nucleus speckles, cytoplasm and endoplasmic reticulum.
Beyond protein targeting, SRP54 plays a role in the proliferation and differentiation of granulocytic cells, in the migration capacity of neutrophils and in exocrine pancreas development. The gene is implicated in severe congenital neutropenia 8. It was previously named signal recognition particle 54kDa, and mouse and rat each have an Srp54 ortholog.
SRP54 recognises signal sequences and drives cotranslational delivery of secretory and membrane proteins to the endoplasmic reticulum.
Its basal GTPase activity and GTP-dependent complex with SRPRA make it a model for studying SRP receptor activation and rearrangement.
SRP54 is involved in granulocyte differentiation and is implicated in severe congenital neutropenia 8.
The protein contributes to neutrophil migration capacity and exocrine pancreas development, relevant to developmental and immune research.
SRP54 is a subunit of the signal recognition particle. It binds the signal sequence of presecretory proteins as they leave the ribosome, then works with the SRP receptor subunit SRPRA through GTP-dependent GTPase activation to deliver them to the endoplasmic reticulum membrane.
The approved HGNC name is signal recognition particle 54, and the previous name was signal recognition particle 54kDa. UniProt lists it as Signal recognition particle subunit SRP54. It is assigned EC 3.6.5.4, reflecting its GTPase activity, and no other aliases are recorded.
SRP54 is implicated in severe congenital neutropenia 8 and has roles in granulocytic differentiation. Mouse and rat both carry an Srp54 ortholog. Check the datasheet of any product for its validated reactivity.
Other signal recognition particle subunits and SRP-named proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P61011; product specifications from Assay Genie datasheets.