PIK3C3
PIK3C3 (phosphatidylinositol 3-kinase catalytic subunit type 3, also known as Vps34 and hVps34) is a lipid kinase of the PI3/PI4-kinase family. It has 1-phosphatidylinositol-3-kinase activity and is linked to endosomal transport and autophagy. Browse Assay Genie's current range of PIK3C3 research reagents below, or read on for the biology behind the target.
PIK3C3 is a lipid kinase that enables 1-phosphatidylinositol-3-kinase activity, and it belongs to the PI3/PI4-kinase family. It is involved in several cellular processes, including early endosome to late endosome transport and macroautophagy. It also acts upstream of or within autophagy and positive regulation of protein lipidation, and it contributes to positive regulation of natural killer cell mediated cytotoxicity. Its roles in membrane trafficking and autophagy make it a frequent subject for researchers studying vesicle biology, and a PIK3C3 antibody or PIK3C3 ELISA kit is a common way to examine its levels in these settings.
The PIK3C3 gene encodes a protein that localises to several compartments. UniProt lists it at the midbody, the late endosome and the autophagosome, a type of cytoplasmic vesicle. NCBI Gene adds that it is located in the autolysosome, late endosome and midbody, and is active at the plasma membrane. This spread of locations fits its involvement in both endosomal transport and autophagic pathways. HGNC places the gene in the phosphatidylinositol 3-kinase family and among the PIK3C3 complex subunits, which indicates that the protein works as part of a larger complex.
The protein is better known to many researchers as Vps34, with hVps34 used for the human protein. Earlier HGNC names include phosphoinositide-3-kinase, class 3, and phosphatidylinositol 3-kinase, catalytic subunit type 3. Mouse and rat both have an ortholog named Pik3c3. In the context of infection, UniProt notes that the kinase activity of PIK3C3 is required for replication of SARS coronavirus-2 (SARS-CoV-2), which has made the enzyme relevant to virology as well as cell biology.
PIK3C3 is involved in macroautophagy and acts upstream of or within autophagy and positive regulation of protein lipidation.
The kinase participates in early endosome to late endosome transport and is found at the late endosome.
Kinase activity of PIK3C3 is required for SARS-CoV-2 replication, making it a host factor of interest in infection studies.
PIK3C3 is involved in positive regulation of natural killer cell mediated cytotoxicity, linking vesicle biology to immune function.
PIK3C3 enables 1-phosphatidylinositol-3-kinase activity. It is involved in early endosome to late endosome transport and macroautophagy, acts upstream of or within autophagy and protein lipidation, and positively regulates natural killer cell mediated cytotoxicity.
PIK3C3 is also called Vps34 or hVps34. Previous HGNC names are phosphoinositide-3-kinase, class 3, and phosphatidylinositol 3-kinase, catalytic subunit type 3. The enzyme carries the EC number 2.7.1.137 and belongs to the PI3/PI4-kinase family.
Mouse and rat both have an ortholog called Pik3c3. UniProt notes that PIK3C3 kinase activity is required for SARS-CoV-2 replication. Check the datasheet of any product for its validated reactivity.
Other phosphoinositide kinases and PI3K family members that are relevant to lipid signalling and membrane trafficking.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8NEB9; product specifications from Assay Genie datasheets.