DHODH
DHODH (dihydroorotate dehydrogenase (quinone)) is a mitochondrial flavoprotein oxidoreductase that catalyses the conversion of dihydroorotate to orotate, using quinone as the electron acceptor. It is required for UMP biosynthesis through the de novo pathway. Browse Assay Genie's current range of DHODH research reagents below, or read on for the biology behind the target.
DHODH catalyses the fourth enzymatic step in de novo pyrimidine biosynthesis: the ubiquinone-mediated oxidation of dihydroorotate to orotate. Quinone serves as the electron acceptor in this reaction. The activity is required for UMP biosynthesis via the de novo pathway, so the enzyme links nucleotide production to the mitochondrial quinone pool.
The DHODH protein is located in the mitochondrion, on the outer surface of the inner mitochondrial membrane. It is classed by HGNC among the flavoproteins and oxidoreductases, and it belongs to the dihydroorotate dehydrogenase family, Type 2 subfamily. The human DHODH gene has orthologs in the mouse and rat, both named Dhodh, which allows the enzyme to be studied in common rodent models.
The enzyme was formerly listed under the name dihydroorotate dehydrogenase, and the current HGNC name, dihydroorotate dehydrogenase (quinone), reflects its use of quinone as the electron acceptor. Because it catalyses a defined step in pyrimidine synthesis at the mitochondrial membrane, DHODH is a useful reference point for work on nucleotide metabolism and mitochondrial enzyme biology. Researchers studying this enzyme may look for a DHODH antibody, a DHODH ELISA kit or recombinant DHODH protein to examine its levels and activity.
DHODH catalyses the fourth step of de novo pyrimidine biosynthesis, making it a key enzyme for studying how cells produce UMP.
Located on the outer surface of the inner mitochondrial membrane, DHODH is a model for quinone-linked oxidation at the mitochondrion.
As a Type 2 member of the dihydroorotate dehydrogenase family, DHODH supports comparative work on flavoprotein redox enzymes.
Because DHODH is required for de novo UMP synthesis, it is relevant to studies of nucleotide supply and metabolic pathway flux.
DHODH catalyses the conversion of dihydroorotate to orotate, with quinone as the electron acceptor. This is the fourth enzymatic step in de novo pyrimidine biosynthesis and is required for UMP biosynthesis via the de novo pathway.
DHODH sits on the outer surface of the inner mitochondrial membrane. The HGNC name is dihydroorotate dehydrogenase (quinone), and the previous name was dihydroorotate dehydrogenase. No alternative gene symbols are listed. UniProt names the protein as mitochondrial.
Yes. Mouse and rat both carry a Dhodh ortholog of the human gene. The protein belongs to the dihydroorotate dehydrogenase family, Type 2 subfamily. Check the datasheet of any product for its validated reactivity.
Other pyrimidine biosynthesis enzymes and nucleotide-metabolism targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q02127; product specifications from Assay Genie datasheets.