SLC12A3
SLC12A3 (solute carrier family 12 member 3, also known as NCCT, NCC and TSC) is an electroneutral sodium and chloride cotransporter that mediates salt reabsorption in the kidney distal convoluted tubule. It also acts as a receptor for the cytokine IL18. Browse Assay Genie's current range of SLC12A3 research reagents below, or read on for the biology behind the target.
SLC12A3 is an electroneutral sodium and chloride ion cotransporter and a key mediator of sodium and chloride reabsorption in the distal convoluted tubules of the kidney. The renal thiazide-sensitive cotransporter is important for electrolyte homeostasis, and it is the target of thiazide diuretics, which are used to treat high blood pressure. Researchers studying this transporter often work with an SLC12A3 antibody, an SLC12A3 ELISA kit or recombinant SLC12A3 protein to examine its expression and function.
The protein localises to the cell membrane, including the apical cell membrane, and belongs to the SLC12A transporter family. The SLC12A3 gene is a member of the HGNC solute carrier family 12 group and is also listed among the microRNA protein coding host genes. Multiple transcript variants encoding different isoforms have been reported. Mouse and rat both carry an orthologous gene, Slc12a3.
Beyond ion transport, SLC12A3 also acts as a receptor for the pro-inflammatory cytokine IL18. In this role it contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2, and it may act either independently of IL18R1 or in a complex with IL18R1.
Mutations in the gene cause Gitelman syndrome, a disease similar to Bartter's syndrome. It is characterised by hypokalaemic alkalosis combined with hypomagnesaemia, low urinary calcium and increased renin activity, with normal blood pressure. The gene was previously named solute carrier family 12 (sodium/chloride transporter), member 3, and the symbols NCCT, NCC and TSC appear in older literature.
SLC12A3 mediates sodium and chloride reabsorption in the distal convoluted tubule, making it central to studies of renal salt handling and electrolyte balance.
As the target of thiazide diuretics used to treat high blood pressure, SLC12A3 is a focus for research on diuretic action.
Mutations in SLC12A3 cause Gitelman syndrome, featuring hypokalaemic alkalosis, hypomagnesaemia, low urinary calcium and raised renin activity with normal blood pressure.
SLC12A3 acts as an IL18 receptor, contributing to IL18-induced production of IFNG, IL6, IL18 and CCL2, independently of or with IL18R1.
SLC12A3 is an electroneutral sodium and chloride cotransporter that mediates salt reabsorption in kidney distal convoluted tubules. It also acts as a receptor for IL18, contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. Check the datasheet of any product for its validated reactivity.
SLC12A3 is also called NCCT, NCC and TSC, and was previously named solute carrier family 12 (sodium/chloride transporter), member 3. It is known as the thiazide-sensitive sodium-chloride cotransporter. Multiple transcript variants encoding different isoforms have been described for the gene.
Yes. Mutations in the SLC12A3 gene cause Gitelman syndrome, which is characterised by hypokalaemic alkalosis, hypomagnesaemia, low urinary calcium and increased renin activity with normal blood pressure. The transporter is also the target of thiazide diuretics used for high blood pressure. Mouse and rat share an ortholog, Slc12a3.
Other SLC12A family cotransporters and further solute carrier targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P55017; product specifications from Assay Genie datasheets.