ATP2A2
ATP2A2, also known as SERCA2, is a P-type calcium ATPase that pumps calcium from the cytosol into the sarcoplasmic or endoplasmic reticulum lumen. It helps regulate the contraction/relaxation cycle, and the gene is also known by the aliases ATP2B and DAR. Browse Assay Genie's current range of ATP2A2 research reagents below, or read on for the biology behind the target.
ATP2A2 encodes sarcoplasmic/endoplasmic reticulum calcium ATPase 2, one of the SERCA Ca(2+)-ATPases. These intracellular pumps sit in the sarcoplasmic or endoplasmic reticulum membrane. The enzyme catalyses the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and it is involved in regulating the contraction/relaxation cycle.
UniProt also assigns SERCA2 a role in Ca(2+) signalling in bone biology. It regulates TNFSF11-mediated Ca(2+) signalling through its interaction with TMEM64. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking, which activates NFATC1 and drives mitochondrial ROS production. These events are critical for TNFSF11-induced CREB1 activation and promote osteoclast differentiation and activation.
The protein belongs to the cation transport ATPase (P-type) family, Type IIA subfamily, and the HGNC places the gene in the group of Ca2+ transporting ATPases. The ATP2A2 gene was previously named ATPase, Ca++ transporting, cardiac muscle, slow twitch 2. Mutations in the gene cause Darier-White disease (keratosis follicularis), an autosomal dominant skin disorder marked by loss of adhesion between epidermal cells and abnormal keratinization. Other mutations have been associated with various forms of muscular dystrophy. Alternative splicing produces multiple transcript variants that encode different isoforms. Mouse and rat both carry an orthologous Atp2a2 gene.
SERCA2 moves calcium into the sarcoplasmic reticulum and helps regulate the contraction/relaxation cycle, making it central to studies of Ca2+ homeostasis.
Through its interaction with TMEM64, SERCA2 drives Ca2+ spiking, NFATC1 activation and mitochondrial ROS production needed for osteoclast generation.
ATP2A2 mutations cause Darier-White disease, a skin disorder involving loss of epidermal cell adhesion and abnormal keratinization.
Other types of ATP2A2 mutations have been associated with various forms of muscular dystrophy, so the pump features in muscle disease research.
ATP2A2 encodes SERCA2, a pump in the sarcoplasmic or endoplasmic reticulum membrane. It hydrolyses ATP to move calcium from the cytosol into the reticulum lumen, and it helps regulate the contraction/relaxation cycle. It also supports TMEM64-linked Ca2+ signalling in osteoclast differentiation.
ATP2A2 is widely called SERCA2, and the aliases ATP2B and DAR also appear. Its full name is ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2. An earlier name was ATPase, Ca++ transporting, cardiac muscle, slow twitch 2. Alternative splicing gives several isoforms.
Mutations in ATP2A2 cause Darier-White disease (keratosis follicularis), and other mutations have been associated with muscular dystrophies. Mouse and rat each have an orthologous Atp2a2 gene. Check the datasheet of any product for its validated reactivity.
Other SERCA isoforms, P-type ATPases and ion-transport proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P16615; product specifications from Assay Genie datasheets.