SPAG5
SPAG5 (sperm associated antigen 5, also known as DEEPEST, MAP126 and hMAP126) is a component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. It also contributes to centriole duplication and to stress responses in non-mitotic cells. Browse Assay Genie's current range of SPAG5 research reagents below, or read on for the biology behind the target.
SPAG5 is an essential component of the mitotic spindle. It is required for chromosome alignment, for the normal timing of sister chromatid segregation and for progression into anaphase, and it helps maintain spindle pole architecture. In complex with SKAP, it promotes stable microtubule-kinetochore attachments. It may also contribute to the regulation of separase activity, and may influence the spindle localisation of AURKA and the localisation of CCNB1 to both spindle and centrosomes. The NCBI Gene summary describes the encoded protein as associated with the mitotic spindle apparatus and possibly involved in the dynamic regulation of spindles.
Beyond the spindle, SPAG5 is involved in centriole duplication. It is required for the centrosomal localisation of CDK5RAP2, CEP152, WDR62 and CEP63, and promotes the centrosomal localisation of CDK2. UniProt records the protein in several compartments: cytoplasm, spindle, spindle pole, kinetochore, midbody, centrosome, centriolar satellite and cytoplasmic granules. The HGNC places the SPAG5 gene in the centriolar satellite protein group.
In non-mitotic cells under stress, SPAG5 inhibits mTORC1 association and recruits the mTORC1 component RPTOR to stress granules. This prevents mTORC1 hyperactivation-induced apoptosis. It may also enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU. The human protein is encoded on chromosome 17, and the mouse ortholog is Spag5. Researchers studying SPAG5 work on mitosis, centrosome biology and cellular stress signalling.
SPAG5 is required for chromosome alignment, correct timing of sister chromatid segregation and progression into anaphase.
In complex with SKAP, SPAG5 promotes stable microtubule-kinetochore attachments, making it a focus for spindle mechanics.
SPAG5 is involved in centriole duplication and is needed for centrosomal localisation of CDK5RAP2, CEP152, WDR62 and CEP63.
Under stress, SPAG5 recruits RPTOR to stress granules, preventing mTORC1 hyperactivation-induced apoptosis in non-mitotic cells.
SPAG5 is an essential mitotic spindle component needed for chromosome alignment, sister chromatid segregation timing and progression into anaphase. It maintains spindle pole architecture, supports centriole duplication, and in stressed non-mitotic cells recruits RPTOR to stress granules, limiting mTORC1-driven apoptosis.
SPAG5 stands for sperm associated antigen 5. Aliases listed are DEEPEST, MAP126 and hMAP126. UniProt names the protein sperm-associated antigen 5 (Q96R06). The gene has no previous names recorded by HGNC, and it belongs to the centriolar satellite protein group.
A mouse ortholog, Spag5, is recorded for the human gene. Researchers working in mouse models should check the datasheet of any product for its validated reactivity. No rat ortholog is given in the data supplied here.
Other sperm associated antigen family members that share the SPAG name, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q96R06; product specifications from Assay Genie datasheets.