SUPT5H
SUPT5H (SPT5 homolog, DSIF elongation factor subunit, also known as SPT5H or SPT5) is a nuclear component of the DSIF complex. It regulates mRNA processing and transcription elongation by RNA polymerase II. Browse Assay Genie's current range of SUPT5H research reagents below, or read on for the biology behind the target.
SUPT5H is a component of the DRB sensitivity-inducing factor complex (DSIF), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT (also called CAP1A). It also acts cooperatively with the negative elongation factor (NELF) complex to enhance transcriptional pausing at sites close to the promoter. Pausing may help assemble an elongation-competent RNA polymerase II complex.
DSIF and NELF promote pausing by inhibiting the elongation factor TFIIS (S-II). TFIIS binds RNA polymerase II at pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleaving blocked transcripts lets transcription resume from a new 3' terminus, which may allow repeated attempts to read through natural pause sites. After phosphorylation by CDK9, DSIF can instead regulate elongation positively. The SUPT5H protein is also required for efficient activation of elongation by the HIV-1 transcriptional activator Tat. At the HIV-1 LTR, DSIF suppresses pausing and blocks premature release of transcripts at terminator sequences.
The SUPT5H gene encodes a nuclear protein of the SPT5 family, located in the nucleoplasm. NCBI Gene annotation lists enzyme binding and protein heterodimerisation activity, a contribution to RNA polymerase inhibitor activity, and involvement in positive regulation of macroautophagy and in regulation of DNA-templated transcription. The mouse ortholog is Supt5h. Earlier names for the gene were suppressor of Ty 5 homolog (S. cerevisiae), and the symbol FLJ34157 has also been used.
SUPT5H is part of DSIF, which regulates RNA polymerase II elongation and, after CDK9 phosphorylation, can switch from pausing to positive elongation control.
Together with the NELF complex, DSIF enhances pausing near the promoter, which may facilitate assembly of an elongation-competent polymerase complex.
DSIF positively regulates mRNA capping by stimulating the guanylyltransferase activity of RNGTT, linking elongation to mRNA processing.
SUPT5H is required for efficient Tat-driven activation of elongation and suppresses pausing and premature transcript release at the HIV-1 LTR.
SUPT5H is a subunit of the DSIF complex, which regulates mRNA processing and RNA polymerase II elongation. DSIF stimulates mRNA capping, works with NELF to enhance promoter-proximal pausing, and after CDK9 phosphorylation can positively regulate elongation.
SUPT5H is also known as SPT5H and SPT5, with the older symbol FLJ34157. UniProt names the protein transcription elongation factor SPT5. Previous gene names include suppressor of Ty 5 homolog (S. cerevisiae). It is the largest subunit of DSIF in the source annotation.
SUPT5H belongs to the SPT5 family and has a mouse ortholog, Supt5h. DSIF is required for efficient activation of elongation by HIV-1 Tat and suppresses pausing in transcripts from the HIV-1 LTR. Check the datasheet of any product for its validated reactivity.
Other transcription elongation factors, DSIF and NELF partners and RNA polymerase II regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O00267; product specifications from Assay Genie datasheets.