CPSF1
CPSF1, also known as CPSF160, is the largest subunit of the cleavage and polyadenylation specificity factor (CPSF) complex. It recognises the AAUAAA signal in pre-mRNA and helps bring about 3'-end cleavage and poly(A) addition. Browse Assay Genie's current range of CPSF1 research reagents below, or read on for the biology behind the target.
CPSF1 (cleavage and polyadenylation specific factor 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, a multisubunit assembly with a central role in the 3-prime processing of pre-mRNAs. The complex recognises the AAUAAA signal sequence in the pre-mRNA and interacts with poly(A) polymerase and other factors to bring about both RNA cleavage and poly(A) synthesis. Within the complex, the CPSF1 protein is involved in the RNA recognition step of the polyadenylation reaction, and it is described as the largest subunit of CPSF.
The CPSF1 gene belongs to the CPSF1 family and sits in the HGNC gene group of cleavage and polyadenylation specific factor subunits. UniProt places the protein in the nucleus, in the nucleoplasm, consistent with its role in nuclear mRNA maturation. HGNC also lists the gene among the microRNA protein coding host genes.
The gene was historically named for the apparent size of its product, and the alias CPSF160 and the previous names 'cleavage and polyadenylation specific factor 1, 160kD subunit' and 'CPSF 160 kDa subunit' reflect this. The mouse ortholog is Cpsf1. Beyond mRNA processing, UniProt notes that CPSF1 may play a role in eye morphogenesis and in the development of retinal ganglion cell projections to the midbrain, although this is described as a possible function.
CPSF1 is a key component of the machinery that cleaves pre-mRNA and adds the poly(A) tail, making it central to studies of transcript maturation.
The protein takes part in recognising the AAUAAA signal sequence, so it is relevant to work on RNA recognition in the polyadenylation reaction.
As the largest CPSF subunit, CPSF1 helps researchers examine how the multisubunit complex interacts with poly(A) polymerase and other factors.
CPSF1 may play a role in eye morphogenesis and in the development of retinal ganglion cell projections to the midbrain.
CPSF1 is a subunit of the CPSF complex, which has a key role in pre-mRNA 3'-end formation. It takes part in the RNA recognition step, recognising the AAUAAA signal and working with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition.
CPSF1 is also known as CPSF160. Earlier names include 'cleavage and polyadenylation specific factor 1, 160kD subunit' and 'CPSF 160 kDa subunit'. UniProt lists the recommended name as cleavage and polyadenylation specificity factor subunit 1, and the protein belongs to the CPSF1 family.
A mouse ortholog, Cpsf1, is recorded for the human gene. The source data do not list a rat ortholog, so check the datasheet of any product for its validated reactivity before choosing a reagent for work in non-human species.
Other cleavage and polyadenylation factors and pre-mRNA 3'-end processing proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q10570; product specifications from Assay Genie datasheets.