POLR1H
POLR1H (RNA polymerase I subunit H, also known as RPA12, ZNRD1, hZR14, HTEX-6, tctex-6 and A12.2) is a core component of RNA polymerase I, the enzyme that synthesises ribosomal RNA precursors. It also helps Pol I monitor the fidelity of transcription. Browse Assay Genie's current range of POLR1H research reagents below, or read on for the biology behind the target.
POLR1H is a core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesises ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Beyond its structural role, the POLR1H protein can mediate Pol I proofreading of the nascent RNA transcript. It anchors into the Pol I active site to monitor transcription fidelity and to cleave mis-incorporated 5'-ribonucleotides.
The POLR1H gene encodes a DNA-directed RNA polymerase I subunit, listed by HGNC in the RNA polymerase subunits gene group. According to the NCBI Gene summary, the encoded protein contains two potential zinc-binding motifs, and alternative splicing produces multiple transcript variants. UniProt places the protein in the nucleus, in the nucleolus, which is consistent with its role in ribosomal RNA synthesis. It belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family, which also includes subunits of the other nuclear RNA polymerases. Mouse and rat each have a Polr1h ortholog.
The gene has had several names. It was formerly called zinc ribbon domain containing 1 (ZNRD1), and the protein is also known as RPA12, A12.2, hZR14, HTEX-6 and tctex-6. RefSeq notes that the protein may play a role in regulating cell proliferation and may be involved in cancer and in human immunodeficiency virus progression. Studies of POLR1H therefore connect basic transcription biology with proliferation and disease research.
POLR1H is a core Pol I subunit, making it relevant to studies of how ribosomal RNA precursors are synthesised in the nucleolus.
The protein anchors in the Pol I active site and can cleave mis-incorporated 5'-ribonucleotides, so it is studied in RNA proofreading.
RefSeq reports that the encoded protein may play a role in the regulation of cell proliferation.
The encoded protein may be involved in cancer and in human immunodeficiency virus progression, according to the NCBI Gene summary.
POLR1H is a core component of RNA polymerase I, which synthesises ribosomal RNA precursors. It can mediate Pol I proofreading, anchoring in the active site to monitor transcription fidelity and cleave mis-incorporated 5'-ribonucleotides from the nascent transcript.
POLR1H is also called RPA12, A12.2, ZNRD1, hZR14, HTEX-6 and tctex-6. Its former HGNC name was zinc ribbon domain containing 1, and UniProt lists the protein as DNA-directed RNA polymerase I subunit RPA12. Alternative splicing gives multiple transcript variants.
Mouse and rat both have a Polr1h ortholog, and the protein belongs to a polymerase family found from archaea to eukaryotes. RefSeq notes possible involvement in cancer and HIV progression; check the datasheet of any product for its validated reactivity.
Other RNA polymerase II and III subunits and related transcription machinery targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9P1U0; product specifications from Assay Genie datasheets.