LARP7
LARP7 (La-related protein 7, also known as HDCMA18P, PIP7S and DKFZP564K112) is a nuclear RNA-binding protein that binds small nuclear RNAs and regulates their processing and function. It is a core component of the 7SK snRNP, a negative regulator of transcription elongation. Browse Assay Genie's current range of LARP7 research reagents below, or read on for the biology behind the target.
LARP7 binds the 7SK small nuclear RNA and acts as a core component of the 7SK ribonucleoprotein (RNP) complex. It recognises the highly conserved 3'-terminal U-rich stretch of 7SK RNA. Within this complex, positive transcription elongation factor b (P-TEFb) is held in a large inactive form, which prevents RNA polymerase II phosphorylation and the subsequent elongation of transcription. On stimulation, LARP7 remains associated with 7SK RNA while P-TEFb is released. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II through interaction with the little elongation complex (LEC).
LARP7 has a second role in RNA processing. It binds U6 snRNAs carrying a 5'-CAGGG-3' sequence motif and associates with a subset of box C/D RNP complexes. By facilitating U6 snRNA loading into these complexes, it promotes U6 snRNA 2'-O-methylation, which is required for mRNA splicing fidelity. U6 snRNA processing is in turn required for spermatogenesis. UniProt places the protein in the nucleoplasm and in the LARP7 family.
The LARP7 gene is grouped by HGNC with the La ribonucleoproteins, RNA binding motif containing proteins and the 7SK snRNP complex. Earlier HGNC names include La ribonucleoprotein domain family, member 7. According to the NCBI Gene summary, the encoded protein in the 7SK snRNP helps inhibit P-TEFb, a cyclin-dependent kinase needed for paused RNA polymerase II to begin elongation. A pseudogene is located on chromosome 3, and alternative splicing yields multiple transcript variants. Mouse and rat both have a Larp7 ortholog.
LARP7 is a core 7SK RNP component that restrains RNA polymerase II elongation by sequestering P-TEFb in an inactive complex.
LARP7 binds distinct snRNAs and regulates their processing, and 7SK RNP activity supports snRNA gene transcription through the little elongation complex.
By promoting U6 snRNA 2'-O-methylation through box C/D RNP loading, LARP7 supports mRNA splicing fidelity.
U6 snRNA processing, in which LARP7 takes part, is required for spermatogenesis, linking this RNA-binding protein to germ cell biology.
LARP7 is an RNA-binding protein that binds 7SK snRNA and forms part of the 7SK RNP complex, which holds P-TEFb inactive and so restrains RNA polymerase II elongation. It also binds U6 snRNA and promotes its 2'-O-methylation, supporting mRNA splicing fidelity.
LARP7 is the HGNC symbol for La ribonucleoprotein 7, transcriptional regulator, also called La-related protein 7. Aliases include HDCMA18P, PIP7S and DKFZP564K112. Earlier names were La ribonucleoprotein domain family, member 7. Alternative splicing of the gene produces multiple transcript variants.
Yes. Mouse and rat each have an ortholog named Larp7, so the gene can be studied in common rodent models. The human protein belongs to the LARP7 family, and the human gene has a pseudogene on chromosome 3. Check the datasheet of any product for its validated reactivity.
Other La-related proteins, P-TEFb components and 7SK snRNP partners with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q4G0J3; product specifications from Assay Genie datasheets.