LRRFIP1
LRRFIP1 (LRR binding FLII interacting protein 1) is a transcriptional repressor of the LRRFIP family that binds GC-rich DNA and also influences Toll-like receptor signalling. It is also known as FLAP-1, FLIIAP1, TRIP, GCF-2, HUFI-1 and GCF2. Browse Assay Genie's current range of LRRFIP1 research reagents below, or read on for the biology behind the target.
LRRFIP1 acts as a transcriptional repressor. It preferentially binds the GC-rich consensus sequence 5'-AGCCCCCGGCG-3' and may regulate expression of TNF, EGFR and PDGFA. Through repression of PDGFA, it may control smooth muscle cell proliferation following artery injury. The protein may also bind double-stranded RNA, which suggests a role beyond DNA-directed transcription control.
LRRFIP1 also has a role in innate immunity. It positively regulates Toll-like receptor (TLR) signalling in response to agonist, probably by competing with the negative regulator FLII for binding to MYD88. NCBI Gene annotation describes DNA-binding transcription repressor activity specific to RNA polymerase II, sequence-specific binding to RNA polymerase II cis-regulatory regions, and protein homodimerisation activity. The protein is assigned to the nucleus and the cytoplasm, and the Alliance of Genome Resources places it in the cytosol.
The LRRFIP1 gene is named for its interaction with the leucine-rich repeat region of flightless I homologue (FLII). Its previous HGNC name was leucine rich repeat (in FLII) interacting protein 1. The alias GCF-2 reflects its binding to GC-rich elements, and mouse and rat each carry an ortholog named Lrrfip1. Researchers study LRRFIP1 in transcriptional control, vascular smooth muscle biology and TLR-driven inflammatory signalling.
LRRFIP1 binds a GC-rich consensus sequence and represses transcription, making it a model for studying RNA polymerase II-specific gene repression.
Repression of PDGFA by LRRFIP1 may control smooth muscle cell proliferation after artery injury.
LRRFIP1 positively regulates TLR signalling, probably by competing with FLII for MYD88 binding.
LRRFIP1 may regulate expression of TNF, EGFR and PDGFA, linking it to inflammatory and growth factor pathways.
LRRFIP1 is a transcriptional repressor that preferentially binds a GC-rich consensus sequence and may regulate TNF, EGFR and PDGFA expression. It may also bind double-stranded RNA. It positively regulates Toll-like receptor signalling, probably by competing with FLII for MYD88 binding.
The HGNC name is LRR binding FLII interacting protein 1, and UniProt lists leucine-rich repeat flightless-interacting protein 1. Aliases include FLAP-1, FLIIAP1, TRIP, GCF-2, HUFI-1 and GCF2. The earlier HGNC name was leucine rich repeat (in FLII) interacting protein 1.
Yes. Mouse and rat both have an ortholog named Lrrfip1. Human LRRFIP1 belongs to the LRRFIP family. Cross-species work should check the datasheet of any product for its validated reactivity.
Other LRRFIP family members, TLR signalling partners and growth factor targets regulated by LRRFIP1.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q32MZ4; product specifications from Assay Genie datasheets.