ATP6V1A
ATP6V1A (ATPase H+ transporting V1 subunit A, also known as Vma1, VA68, VPP2, ATP6A1 and ATP6V1A1) encodes the catalytic subunit A of the vacuolar ATPase, the enzyme that acidifies intracellular organelles in eukaryotic cells. It forms part of the V1 domain, which contains the ATP catalytic site. Browse Assay Genie's current range of ATP6V1A research reagents below, or read on for the biology behind the target.
The ATP6V1A protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. This organelle acidification is necessary for processes such as protein sorting, zymogen activation, receptor-mediated endocytosis and the generation of the synaptic vesicle proton gradient. V-ATPase is built from a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits, plus the C, D, E, F and H subunits, and it carries the ATP catalytic site. The V0 domain contains five different subunits: a, c, c', c" and d. ATP6V1A provides the A subunits of V1.
The ATP6V1A gene encodes one of two V1 domain A subunit isoforms and is found in all tissues. Many V1 and V0 subunits have additional isoforms, encoded by multiple genes or by alternatively spliced transcript variants, and transcript variants of this gene arise through alternative polyadenylation. UniProt places the protein in the cytoplasm and cytosol, in secretory vesicles, in clathrin-coated vesicle membranes and at the lysosome membrane. It belongs to the ATPase alpha/beta chains family, and HGNC lists it among the V-type ATPase subunits and the ATPase F1/V1 alpha/A and beta/B subunit family. The mouse ortholog is Atp6v1a.
Earlier gene names described it as the lysosomal (vacuolar proton pump) alpha polypeptide, 70kD, isoform 1. In the context of microbial infection, UniProt notes that ATP6V1A plays an important role in virion uncoating during Rabies virus replication after membrane fusion. It participates in the dissociation of incoming viral matrix M proteins through direct interaction. Researchers studying ATP6V1A often work with an ATP6V1A antibody or ATP6V1A ELISA kit to examine its expression and role in V-ATPase assembly.
ATP6V1A supplies the catalytic A subunits of V-ATPase, which acidifies intracellular organelles and so supports many compartment-dependent processes.
V-ATPase-dependent acidification is necessary for receptor-mediated endocytosis and protein sorting, making this subunit relevant to trafficking studies.
V-ATPase activity generates the synaptic vesicle proton gradient, linking the A subunit to studies of vesicle function.
ATP6V1A participates in Rabies virus uncoating by interacting directly with incoming viral matrix M proteins after membrane fusion.
ATP6V1A encodes the catalytic A subunit in the V1 domain of V-ATPase, which holds the ATP catalytic site. V-ATPase acidifies eukaryotic intracellular organelles, a process needed for protein sorting, zymogen activation, receptor-mediated endocytosis and synaptic vesicle proton gradient generation.
Aliases and previous symbols include Vma1, VA68, VPP2, ATP6A1 and ATP6V1A1. UniProt calls it V-type proton ATPase catalytic subunit A. Older names describe it as the lysosomal (vacuolar proton pump) alpha polypeptide, 70kD, isoform 1. Transcript variants arise through alternative polyadenylation.
The gene encodes one of two V1 domain A subunit isoforms and is found in all tissues. The mouse ortholog is Atp6v1a. The protein belongs to the ATPase alpha/beta chains family; check the datasheet of any product for its validated reactivity.
Other ATPases, V-ATPase-associated proteins and ion-transport targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P38606; product specifications from Assay Genie datasheets.