AMOT
AMOT (angiomotin, also known as KIAA1071) is a member of the motin family of angiostatin-binding proteins. It supports tight junction maintenance and regulates endothelial cell migration and tube formation. Browse Assay Genie's current range of AMOT research reagents below, or read on for the biology behind the target.
AMOT plays a central role in tight junction maintenance. It does so through a complex with ARHGAP17, which regulates the uptake of polarity proteins at tight junctions. The protein also appears to regulate endothelial cell migration and tube formation, and may contribute to the assembly of endothelial cell-cell junctions.
AMOT also acts as a repressor of transcription of target genes by YAP1 and WWTR1/TAZ. This is potentially through regulation of Hippo signalling-mediated phosphorylation of YAP1, which leads to its recruitment to tight junctions. It therefore links junctional organisation with Hippo pathway output.
The AMOT gene belongs to the motin family of angiostatin-binding proteins, which are characterised by conserved coiled-coil domains and C-terminal PDZ-binding motifs. According to the NCBI Gene summary, the encoded protein is expressed predominantly in endothelial cells of placental capillaries and larger vessels. There it may mediate the inhibitory effect of angiostatin on tube formation and on the migration of endothelial cells towards growth factors during the formation of new blood vessels. Alternative splicing produces multiple transcript variants that encode different isoforms. The mouse ortholog is Amot, and the human gene is also listed under the earlier designation KIAA1071. HGNC classifies it among microRNA protein-coding host genes.
AMOT, with ARHGAP17, helps maintain tight junctions by regulating the uptake of polarity proteins.
AMOT appears to regulate endothelial cell migration and tube formation, and may mediate the inhibitory effect of angiostatin.
AMOT represses YAP1 and WWTR1/TAZ transcriptional activity, potentially by promoting YAP1 recruitment to tight junctions.
AMOT may play a role in assembling endothelial cell-cell junctions, relevant to vascular barrier and vessel formation studies.
AMOT plays a central role in tight junction maintenance through a complex with ARHGAP17, regulating the uptake of polarity proteins. It also appears to regulate endothelial cell migration and tube formation, and represses YAP1 and WWTR1/TAZ-driven transcription.
AMOT stands for angiomotin and is also known as KIAA1071. It belongs to the motin family of angiostatin-binding proteins. Alternative splicing of the gene results in multiple transcript variants that encode different isoforms.
A mouse ortholog, Amot, is recorded for the human gene. Check the datasheet of any product for its validated reactivity. AMOT belongs to the angiomotin family, which also includes related proteins such as AMOTL1 and AMOTL2.
Other angiomotin family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q4VCS5; product specifications from Assay Genie datasheets.