SKP2
SKP2 (S-phase kinase-associated protein 2, also known as FBXL1, FBL1 and p45) is an F-box protein that acts as a substrate-recognition subunit of SCF ubiquitin ligase complexes. It is linked to S-phase cell cycle control and is established as a protooncogene in lymphoma. Browse Assay Genie's current range of SKP2 research reagents below, or read on for the biology behind the target.
SKP2 is one of the four subunits of the SCF (SKP1-cullin-F-box) ubiquitin protein ligase complex, which functions in phosphorylation-dependent ubiquitination. Within the complex, the F-box protein provides substrate specificity. SKP2 specifically recognises phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also called p27 or KIP1), predominantly in S phase, and it interacts with S-phase kinase-associated protein 1 (SKP1, or p19). It is described as an essential element of the cyclin A-CDK2 S-phase kinase. UniProt also annotates a role in the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, giving the protein antiviral activity towards that virus.
The SKP2 gene encodes a member of the F-box protein family, which is characterised by an F-box motif of approximately 40 amino acids. F-box proteins fall into three classes: Fbws with WD-40 domains, Fbls with leucine-rich repeats, and Fbxs with other protein-protein interaction modules or no recognisable motif. SKP2 belongs to the Fbls class and, in addition to its F-box, contains 10 tandem leucine-rich repeats. UniProt lists the protein in the cytoplasm and nucleus. Alternative splicing of the gene generates three transcript variants that encode different isoforms.
HGNC places SKP2 in the groups of F-box and leucine rich repeat proteins and E3 ubiquitin ligases. Earlier names include S-phase kinase-associated protein 2 (p45), and the symbols FBXL1 and FBL1 have also been used. The gene is established as a protooncogene causally involved in the pathogenesis of lymphomas, which makes SKP2 a frequent subject in studies of cell cycle regulation and cancer biology. The mouse ortholog is Skp2.
SKP2 recognises phosphorylated p27 (CDKN1B) mainly in S phase and is an essential element of the cyclin A-CDK2 S-phase kinase.
As an Fbl-class F-box protein, SKP2 provides substrate recognition within SCF (SKP1-cullin-F-box) complexes that mediate phosphorylation-dependent ubiquitination.
The SKP2 gene is established as a protooncogene causally involved in the pathogenesis of lymphomas, so it is studied in cancer models.
SKP2 drives proteasomal degradation of hepatitis C virus non-structural protein 5A, giving it antiviral activity towards that virus.
SKP2 is the substrate-recognition F-box subunit of an SCF ubiquitin ligase complex. It specifically recognises phosphorylated p27 (CDKN1B, KIP1), mainly in S phase, and interacts with SKP1. It also promotes proteasomal degradation of hepatitis C virus NS5A, giving antiviral activity.
SKP2 is also known as FBXL1, FBL1 and p45, and its full name is S-phase kinase-associated protein 2. Earlier HGNC names include the p45 label and the E3 ubiquitin protein ligase designation. Alternative splicing generates three transcript variants that encode different isoforms.
The SKP2 gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. A mouse ortholog, Skp2, exists. Check the datasheet of any product for its validated reactivity before using it in a particular species.
SKP1, the SCF partner of SKP2, and other proteins involved in ubiquitin ligase and cell cycle research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q13309; product specifications from Assay Genie datasheets.