RAD54L2
RAD54L2 (RAD54 like 2) is a nuclear helicase of the SNF2/RAD54 family, also known as Helicase ARIP4, ARIP4, KIAA0809 and SRISNF2L. It modulates androgen receptor-dependent transactivation and is linked to R-loop processing during transcription. Browse Assay Genie's current range of RAD54L2 research reagents below, or read on for the biology behind the target.
RAD54L2 is a helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. It is proposed to stimulate the release of RNA polymerase II that has paused near the transcription start sites of AR-targeted genes. It may also unwind R-loops formed at these sites, where the short RNA strand nascently synthesised by the paused polymerase hybridises with the DNA template.
The protein may additionally stimulate double-strand break formation by TOP2B, which appears important for transcriptional output. Despite belonging to a family of chromatin-associated enzymes, it is not able to remodel mononucleosomes in vitro. The NCBI Gene summary describes DNA/RNA helicase activity and ubiquitin protein ligase activity, involvement in R-loop processing and transcription elongation by RNA polymerase II, and a location in chromatin.
The RAD54L2 gene encodes a protein of the SNF2/RAD54 helicase family, classed by HGNC in the SNF2 related family. The RAD54L2 protein localises to the nucleus and chromosome. The official name, RAD54 like 2, was previously written RAD54 like 2 (S. cerevisiae), and the gene has also been catalogued as KIAA0809, SRISNF2L and ARIP4. The mouse ortholog is Rad54l2. Researchers studying RAD54L2 will often consider it alongside androgen receptor signalling, transcription elongation and R-loop biology.
RAD54L2 modulates AR-dependent transactivation in a promoter-dependent manner, making it relevant to studies of AR-targeted gene regulation.
RAD54L2 may stimulate release of paused RNA polymerase II near transcription start sites and is involved in elongation by the polymerase.
RAD54L2 is implicated in R-loop processing, potentially unwinding RNA-DNA hybrids formed by paused RNA polymerase II at AR-targeted genes.
RAD54L2 may promote TOP2B-mediated double-strand break formation linked to transcriptional output, and is located in chromatin.
RAD54L2 is a helicase that modulates androgen receptor-dependent transactivation in a promoter-dependent manner. It may release paused RNA polymerase II, unwind R-loops at AR-targeted genes and stimulate TOP2B-dependent double-strand break formation. It cannot remodel mononucleosomes in vitro.
RAD54L2 is also called Helicase ARIP4 (the UniProt protein name), ARIP4, KIAA0809 and SRISNF2L. HGNC lists its full name as RAD54 like 2, previously RAD54 like 2 (S. cerevisiae). Check the datasheet of any product for its validated reactivity.
Yes, a mouse ortholog, Rad54l2, is recognised. The protein belongs to the SNF2/RAD54 helicase family, and the human gene name reflects its original similarity to the yeast RAD54 gene. Check the datasheet of any product for its validated reactivity.
Other RAD-family proteins involved in DNA repair, checkpoint signalling and chromatin biology with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y4B4; product specifications from Assay Genie datasheets.