PIGL
PIGL (phosphatidylinositol glycan anchor biosynthesis class L, also written PIG-L) is an endoplasmic reticulum enzyme that catalyses the second step of glycosylphosphatidylinositol (GPI) biosynthesis. It acts as an N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase. Browse Assay Genie's current range of PIGL research reagents below, or read on for the biology behind the target.
PIGL catalyses the second step of glycosylphosphatidylinositol (GPI) biosynthesis: the de-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol (GlcNAc-PI). This reaction follows the addition of N-acetylglucosamine to phosphatidylinositol and prepares the intermediate for the steps that complete the GPI anchor. The enzyme is classified under EC 3.5.1.89 and is known by the protein name N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase.
The PIGL gene encodes the PIGL protein, which belongs to the PIGL family. UniProt records its location as the endoplasmic reticulum membrane, and the NCBI Gene summary notes that work on a similar rat enzyme suggests localisation to the endoplasmic reticulum. Mouse and rat both have a Pigl ortholog, which allows the enzyme to be studied in common model systems.
In HGNC, PIGL sits in the gene group for phosphatidylinositol glycan anchor biosynthesis, alongside other genes that contribute to building the GPI anchor. It is also listed within the group of microRNA protein coding host genes. The approved name, phosphatidylinositol glycan anchor biosynthesis class L, replaced the earlier name phosphatidylinositol glycan, class L, and the symbol PIG-L is still used as an alias. Researchers working with PIGL, whether through the PIGL gene, the PIGL protein or antibody-based detection, typically do so in the context of GPI anchor assembly in the endoplasmic reticulum.
PIGL carries out the second step of GPI biosynthesis, making it a defined point for studying how the anchor is assembled.
As an endoplasmic reticulum membrane enzyme, PIGL is relevant to work on where and how early GPI intermediates are processed.
PIGL converts GlcNAc-PI by de-N-acetylation under EC 3.5.1.89, offering a focused model for this enzyme activity.
Mouse and rat Pigl orthologs support comparative studies of GPI pathway enzymes across species.
PIGL is an N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase. It catalyses the second step of glycosylphosphatidylinositol (GPI) biosynthesis, the de-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol (GlcNAc-PI). UniProt places the protein in the endoplasmic reticulum membrane.
The HGNC name is phosphatidylinositol glycan anchor biosynthesis class L, with the alias PIG-L and the previous name phosphatidylinositol glycan, class L. UniProt names the protein N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase, and the enzyme is assigned EC 3.5.1.89.
Orthologs of the human gene exist in mouse and rat, both named Pigl. The NCBI Gene summary also notes that study of a similar rat enzyme suggests endoplasmic reticulum localisation. Check the datasheet of any product for its validated reactivity.
Other phosphatidylinositol glycan anchor biosynthesis enzymes and GPI pathway targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9Y2B2; product specifications from Assay Genie datasheets.