MPO
MPO (myeloperoxidase) is a heme protein synthesised during myeloid differentiation and is the major component of neutrophil azurophilic granules. It produces hypohalous acids that are central to the microbicidal activity of neutrophils. Browse Assay Genie's current range of MPO research reagents below, or read on for the biology behind the target.
MPO is a heme-containing peroxidase classified under EC 1.11.2.2. It generates hypohalous acids, the reactive products that underpin the microbicidal activity of neutrophils. The catalytic heme is the site of peroxidase activity, and in the context of microbial infection this activity can be blocked: the Staphylococcus aureus peroxidase inhibitor SPIN prevents substrate access to the catalytic heme.
The MPO gene encodes a single-chain precursor that is subsequently cleaved into a light chain and a heavy chain. The mature MPO protein is a tetramer composed of two light chains and two heavy chains. It is synthesised during myeloid differentiation and forms the major component of neutrophil azurophilic granules. UniProt records subcellular locations including the lysosome, cytolytic granule, phagosome and secreted compartments, as well as the nucleus and chromosome. The mouse ortholog is Mpo.
MPO belongs to the heme peroxidase family, in the XPO subfamily of the peroxidase family. Its role in neutrophil antimicrobial defence makes the MPO protein a common reference point for studies of myeloid cells, granule biology and host-pathogen interaction.
MPO is the major component of neutrophil azurophilic granules, making it a defining protein for studying neutrophil granule content and function.
MPO is synthesised during myeloid differentiation, so it is relevant to research on how myeloid cells develop and mature.
MPO produces hypohalous acids central to the microbicidal activity of neutrophils, supporting studies of host killing of microbes.
The S. aureus inhibitor SPIN blocks substrate access to the MPO catalytic heme, providing a model for how pathogens counter peroxidase activity.
MPO is a heme peroxidase that produces hypohalous acids, which are central to the microbicidal activity of neutrophils. It is the major component of neutrophil azurophilic granules and is synthesised during myeloid differentiation. Check the datasheet of any product for its validated reactivity.
MPO is produced as a single-chain precursor that is cleaved into a light chain and a heavy chain. The mature enzyme is a tetramer of two light chains and two heavy chains, with a catalytic heme at the peroxidase active site.
A mouse ortholog, Mpo, is recorded for the human MPO gene. In microbial infection, the Staphylococcus aureus peroxidase inhibitor SPIN inhibits MPO peroxidase activity by blocking substrate access to the catalytic heme.
Other heme peroxidases, neutrophil granule proteins and myeloid markers studied alongside MPO.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P05164; product specifications from Assay Genie datasheets.